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Absolute yeast mitochondrial proteome quantification reveals trade-off between biosynthesis and energy generation during diauxic shift

Artikel i vetenskaplig tidskrift
Författare Francesca Di Bartolomeo
C. Malina
K. Campbell
M. Mormino
Johannes Fuchs
Egor Vorontsov
Claes M Gustafsson
J. Nielsen
Publicerad i Proceedings of the National Academy of Sciences of the United States of America
Volym 117
Nummer/häfte 13
Sidor 7524-7535
ISSN 0027-8424
Publiceringsår 2020
Publicerad vid Institutionen för biomedicin
Core Facilities, Proteomics
Sidor 7524-7535
Språk en
Länkar dx.doi.org/10.1073/pnas.1918216117
Ämnesord mitochondria, absolute proteomics, Saccharomyces cerevisiae, diauxic, shift, inner membrane, gene, phosphatidylethanolamine, cardiolipin, biogenesis, morphology, proteins, dynamics, insights, density, Science & Technology - Other Topics
Ämneskategorier Biologiska vetenskaper

Sammanfattning

Saccharomyces cerevisiae constitutes a popular eukaryal model for research on mitochondrial physiology. Being Crabtree-positive, this yeast has evolved the ability to ferment glucose to ethanol and respire ethanol once glucose is consumed. Its transition phase from fermentative to respiratory metabolism, known as the diauxic shift, is reflected by dramatic rearrangements of mitochondrial function and structure. To date, the metabolic adaptations that occur during the diauxic shift have not been fully characterized at the organelle level. In this study, the absolute proteome of mitochondria was quantified alongside precise parametrization of biophysical properties associated with the mitochondrial network using state-of-the-art optical-imaging techniques. This allowed the determination of absolute protein abundances at a subcellular level. By tracking the transformation of mitochondrial mass and volume, alongside changes in the absolute mitochondrial proteome allocation, we could quantify how mitochondria balance their dual role as a biosynthetic hub as well as a center for cellular respiration. Furthermore, our findings suggest that in the transition from a fermentative to a respiratory metabolism, the diauxic shift represents the stage where major structural and functional reorganizations in mitochondrial metabolism occur. This metabolic transition, initiated at the mitochondria level, is then extended to the rest of the yeast cell.

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