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Vimentin Phosphorylation Is Required for Normal Cell Division of Immature Astrocytes

Artikel i vetenskaplig tidskrift
Författare Yolanda de Pablo
Pavel Marasek
A. Pozo-Rodrigalvarez
Ulrika Wilhelmsson
M. Inagaki
M. Pekna
Milos Pekny
Publicerad i Cells
Volym 8
Nummer/häfte 9
Publiceringsår 2019
Publicerad vid Institutionen för neurovetenskap och fysiologi, sektionen för klinisk neurovetenskap
Språk en
Länkar dx.doi.org/10.3390/cells8091016
Ämnesord intermediate filaments, nanofilaments, vimentin, vimentin accumulations, GFAP, astrocytes, fibrillary acidic protein, rho-associated kinase, intermediate-filaments, reactive astrocytes, cleavage furrow, mice, deficient, aurora-b, mitochondrial, dynamics, sites, Cell Biology
Ämneskategorier Neurovetenskaper

Sammanfattning

Vimentin (VIM) is an intermediate filament (nanofilament) protein expressed in multiple cell types, including astrocytes. Mice with VIM mutations of serine sites phosphorylated during mitosis (VIMSA/SA) show cytokinetic failure in fibroblasts and lens epithelial cells, chromosomal instability, facilitated cell senescence, and increased neuronal differentiation of neural progenitor cells. Here we report that in vitro immature VIMSA/SA astrocytes exhibit cytokinetic failure and contain vimentin accumulations that co-localize with mitochondria. This phenotype is transient and disappears with VIMSA/SA astrocyte maturation and expression of glial fibrillary acidic protein (GFAP); it is also alleviated by the inhibition of cell proliferation. To test the hypothesis that GFAP compensates for the effect of VIMSA/SA in astrocytes, we crossed the VIMSA/SA and GFAP(-/-) mice. Surprisingly, the fraction of VIMSA/SA immature astrocytes with abundant vimentin accumulations was reduced when on GFAP(-/-) background. This indicates that the disappearance of vimentin accumulations and cytokinetic failure in mature astrocyte cultures are independent of GFAP expression. Both VIMSA/SA and VIM(SA/SA)GFAP(-/-) astrocytes showed normal mitochondrial membrane potential and vulnerability to H2O2, oxygen/glucose deprivation, and chemical ischemia. Thus, mutation of mitotic phosphorylation sites in vimentin triggers formation of vimentin accumulations and cytokinetic failure in immature astrocytes without altering their vulnerability to oxidative stress.

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