Till sidans topp

Sidansvarig: Webbredaktion
Sidan uppdaterades: 2012-09-11 15:12

Tipsa en vän
Utskriftsversion

Oral vancomycin treatment… - Göteborgs universitet Till startsida
Webbkarta
Till innehåll Läs mer om hur kakor används på gu.se

Oral vancomycin treatment does not alter markers of postprandial inflammation in lean and obese subjects

Artikel i vetenskaplig tidskrift
Författare G. J. Bakker
J. G. Schnitzler
S. Bekkering
N. C. Clercq
A. M. Koopen
A. V. Hartstra
E. C. E. Meessen
T. P. Scheithauers
M. Winkelmeijer
G. M. Dallinga-Thie
P. D. Cani
E. M. Kemper
M. R. Soeters
J. Kroon
A. K. Groen
D. H. van Raalte
H. Herrema
Max Nieuwdorp
Publicerad i Physiological Reports
Volym 7
Nummer/häfte 16
ISSN 2051-817X
Publiceringsår 2019
Publicerad vid Wallenberglaboratoriet
Institutionen för medicin, avdelningen för molekylär och klinisk medicin
Språk en
Länkar dx.doi.org/10.14814/phy2.14199
Ämnesord Bacterial endotoxins, bacterial translocation, inflammation, obesity, lipopolysaccharide-binding protein, augments monocyte responses, gut, microbiota, systemic inflammation, circulating monocytes, inhibitory-activity, severe sepsis, endotoxemia, fat, lps, Physiology
Ämneskategorier Fysiologi

Sammanfattning

Intake of a high-fat meal induces a systemic inflammatory response in the postprandial which is augmented in obese subjects. However, the underlying mechanisms of this response have not been fully elucidated. We aimed to assess the effect of gut microbiota modulation on postprandial inflammatory response in lean and obese subjects. Ten lean and ten obese subjects with metabolic syndrome received oral vancomycin 500 mg four times per day for 7 days. Oral high-fat meal tests (50 g fat/m(2) body surface area) were performed before and after vancomycin intervention. Gut microbiota composition, leukocyte counts, plasma lipopolysaccharides (LPS), LPS-binding protein (LBP), IL-6 and MCP-1 concentrations and monocyte CCR2 and cytokine expression were determined before and after the high-fat meal. Oral vancomycin treatment resulted in profound changes in gut microbiota composition and significantly decreased bacterial diversity in both groups (phylogenetic diversity pre- versus post-intervention: lean, 56.9 +/- 7.8 vs. 21.4 +/- 6.6, P < 0.001; obese, 53.9 +/- 7.8 vs. 21.0 +/- 5.9, P < 0.001). After intervention, fasting plasma LPS significantly increased (lean, median [IQR] 0.81 [0.63-1.45] EU/mL vs. 2.23 [1.33-3.83] EU/mL, P = 0.017; obese, median [IQR] 0.76 [0.45-1.03] EU/mL vs. 1.44 [1.11-4.24], P = 0.014). However, postprandial increases in leukocytes and plasma LPS were unaffected by vancomycin in both groups. Moreover, we found no changes in plasma LBP, IL-6 and MCP-1 or in monocyte CCR2 expression. Despite major vancomycin-induced disruption of the gut microbiota and increased fasting plasma LPS, the postprandial inflammatory phenotype in lean and obese subjects was unaffected in this study.

Sidansvarig: Webbredaktion|Sidan uppdaterades: 2012-09-11
Dela:

På Göteborgs universitet använder vi kakor (cookies) för att webbplatsen ska fungera på ett bra sätt för dig. Genom att surfa vidare godkänner du att vi använder kakor.  Vad är kakor?