Till sidans topp

Sidansvarig: Webbredaktion
Sidan uppdaterades: 2012-09-11 15:12

Tipsa en vän
Utskriftsversion

Vagococcus bubulae sp. no… - Göteborgs universitet Till startsida
Webbkarta
Till innehåll Läs mer om hur kakor används på gu.se

Vagococcus bubulae sp. nov., isolated from ground beef, and Vagococcus vulneris sp. nov., isolated from a human foot wound

Artikel i vetenskaplig tidskrift
Författare P. L. Shewmaker
A. M. Whitney
C. A. Gulvik
B. Humrighouse
J. Gartin
H. Moura
J. R. Barr
Edward R.B. Moore
Roger Karlsson
T. C. A. Pinto
L. M. Teixeira
Publicerad i International Journal of Systematic and Evolutionary Microbiology
Volym 69
Nummer/häfte 8
Sidor 2268-2276
ISSN 1466-5026
Publiceringsår 2019
Publicerad vid Institutionen för biomedicin, avdelningen för infektionssjukdomar
Sidor 2268-2276
Språk en
Länkar dx.doi.org/10.1099/ijsem.0.003459
Ämnesord taxonomy, Firmicutes, Vagococcus, Vagococcus bubulae, Vagococcus vulneris, gram-positive cocci, gene, identification, enterococcus, classification, streptococcus, algorithm, bacteria, strains, rpob, Microbiology
Ämneskategorier Infektionsmedicin

Sammanfattning

Two unusual catalase-negative, Gram-stain-positive, Vagococcus-like isolates that were referred to the CDC Streptococcus Laboratory for identification are described. Strain SS1994(T) was isolated from ground beef and strain SS1995(T) was isolated from a human foot wound. Comparative 16S rRNA gene sequence analysis of isolates SS1994(T) and SS1995(T) against Vagococcus type strain sequences supported their inclusion in the genus Vagococcus. Strain SS1994(T) showed high sequence similarity (>97.0 %) to the two most recently proposed species, Vagococcus martis (99.2 %) and Vagococcus teuberi (99.0 %) followed by Vagococcus penaei (98.8 %), strain SS1995(T) (98.6 %), Vagococcus camiphilus (98.0 %), Vagococcus acidifermentans (98.0 %) and Vagococcus fluvialis (97.9 %). The 16S rRNA gene sequence of strain SS1995(T) was most similar to V. penaei (99.1 %), followed by SS1994(T) (98.6%), V. martis (98.4 %), V. teuberi (98.1 %), V. acidifermentans (97.8 %), and both V. camiphilus and V. fluvialis (97.5 %). A polyphasic taxonomic study using conventional biochemical and the rapid ID 32 STREP system, MALDI-TOF MS, cell fatty acid analysis, pairwise sequence comparisons of the 16S rRNA, rpoA, rpoB, pheS and groL genes, and comparative core and whole genome sequence analyses revealed that strains SS1994(T) and SS1995(T) were two novel Vagococcus species. The novel taxonomic status of the two isolates was confirmed with core genome phylogeny, average nucleotide identity <84 %and in silico DNA-DNA hybridization <28 %to any other Vagococcus species. The names Vagococcus bubulae SS1994 (T)=(CCUG 70831(T) =LMG 30164(T)) and Vagococcus vulneris SS1995(T)=(CCUG 70832(T) =LMG 30165(T)) are proposed.

Sidansvarig: Webbredaktion|Sidan uppdaterades: 2012-09-11
Dela:

På Göteborgs universitet använder vi kakor (cookies) för att webbplatsen ska fungera på ett bra sätt för dig. Genom att surfa vidare godkänner du att vi använder kakor.  Vad är kakor?