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Expression of calcification-related ion transporters during blue mussel larval development

Artikel i vetenskaplig tidskrift
Författare Kirti Ramesh
T. Yarra
M. S. Clark
U. John
F. Melzner
Publicerad i Ecology and Evolution
Volym 9
Nummer/häfte 12
Sidor 7157-7172
ISSN 2045-7758
Publiceringsår 2019
Publicerad vid Sven Lovén centrum för marina vetenskaper
Sidor 7157-7172
Språk en
Länkar dx.doi.org/10.1002/ece3.5287
Ämnesord biomineralization, bivalves, gene expression, larvae, transcription factor-2 runx2, pearl oyster, gene-expression, seawater, acidification, ocean acidification, pinctada-fucata, mytilus-edulis, protein, shell, genome, Environmental Sciences & Ecology, Evolutionary Biology
Ämneskategorier Marin ekologi

Sammanfattning

The physiological processes driving the rapid rates of calcification in larval bivalves are poorly understood. Here, we use a calcification substrate-limited approach (low dissolved inorganic carbon, C-T) and mRNA sequencing to identify proteins involved in bicarbonate acquisition during shell formation. As a secondary approach, we examined expression of ion transport and shell matrix proteins (SMPs) over the course of larval development and shell formation. We reared four families of Mytilus edulis under ambient (ca. 1865 mu mol/kg) and low C-T (ca. 941 mu mol/kg) conditions and compared expression patterns at six developmental time points. Larvae reared under low C-T exhibited a developmental delay, and a small subset of contigs was differentially regulated between ambient and low C-T conditions. Of particular note was the identification of one contig encoding an anion transporter (SLC26) which was strongly upregulated (2.3-2.9 fold) under low C-T conditions. By analyzing gene expression profiles over the course of larval development, we are able to isolate sequences encoding ion transport and SMPs to enhance our understanding of cellular pathways underlying larval calcification processes. In particular, we observe the differential expression of contigs encoding SLC4 family members (sodium bicarbonate cotransporters, anion exchangers), calcium-transporting ATPases, sodium/calcium exchangers, and SMPs such as nacrein, tyrosinase, and transcripts related to chitin production. With a range of candidate genes, this work identifies ion transport pathways in bivalve larvae and by applying comparative genomics to investigate temporal expression patterns, provides a foundation for further studies to functionally characterize the proteins involved in larval calcification.

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