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Microbial fermentation of flaxseed fibers modulates the transcriptome of GPR41-expressing enteroendocrine cells and protects mice against diet-induced obesity

Artikel i vetenskaplig tidskrift
Författare Tulika Arora
O. Rudenko
K. L. Egerod
A. S. Husted
Petia Kovatcheva-Datchary
Rozita Akrami
M. Kristensen
T. W. Schwartz
F. Bäckhed
Publicerad i American Journal of Physiology - Endocrinology and Metabolism
Volym 316
Nummer/häfte 3
Sidor E453-E463
ISSN 0193-1849
Publiceringsår 2019
Publicerad vid Wallenberglaboratoriet
Sidor E453-E463
Språk en
Länkar dx.doi.org/10.1152/ajpendo.00391.20...
Ämnesord Fermentation, Flaxseed fibers, G protein-coupled receptor 41, Microbiome, Short-chain fatty acids
Ämneskategorier Invärtesmedicin

Sammanfattning

Dietary fibers, an integral part of the human diet, require the enzymatic activity of the gut microbiota for complete metabolism into short-chain fatty acids (SCFAs). SCFAs are important modulators of host metabolism and physiology and act in part as signaling molecules by activating G protein-coupled receptors (GPCRs), such as GPR41. Flaxseed fibers improve metabolism in rodents and mice, but their fermentation profiles, effects on enteroendocrine cells, and associated metabolic benefits are unknown. We fed GPR41-red fluorescent protein mice, an enteroendocrine reporter mouse strain, chow, high-fat diet (HFD), or HFD supplemented either with 10% nonfermentable fiber cellulose or fermentable flaxseed fibers for 12 wk to assess changes in cecal gut microbiota, enteroendocrine cell transcriptome in the ileum and colon, and physiological parameters. We observed that flaxseed fibers restructured the gut microbiota and promoted proliferation of the genera Bifidobacterium and Akkermansia compared with HFD. The shifts in cecal bacterial composition restored levels of the SCFAs butyrate similar to the chow diet, resulting in colonic but not ileal enteroendocrine cell transcriptional changes in genes related to cell cycle, mRNA, and protein transport compared with HFD. Consistent with the effects on enteroendocrine functions, flaxseed fibers also protected mice from diet-induced obesity, potentially by preventing a reduction in energy expenditure induced by an HFD. Our study shows that flaxseed fibers alter cecal microbial ecology, are fermented to SCFAs in the cecum, and modulate enteroendocrine cell transcriptome in the colon, which may contribute to their metabolically favorable phenotype. © 2019 the American Physiological Society.

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