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E-cadherin: a differentiation marker in thyroid malignancies.

Artikel i vetenskaplig tidskrift
Författare G Brabant
C Hoang-Vu
Y Cetin
H Dralle
G Scheumann
Johan Mölne
G Hansson
S Jansson
L E Ericson
M Nilsson
Publicerad i Cancer research
Volym 53
Nummer/häfte 20
Sidor 4987-93
ISSN 0008-5472
Publiceringsår 1993
Publicerad vid Institutionen för laboratoriemedicin , Avdelningen för patologi
Sidor 4987-93
Språk en
Länkar www.ncbi.nlm.nih.gov/entrez/query.f...
Ämnesord Adenoma, metabolism, pathology, Biomarkers, Tumor, analysis, Blotting, Northern, Cadherins, analysis, biosynthesis, Carcinoma, Papillary, metabolism, pathology, Cell Differentiation, Fluorescent Antibody Technique, Humans, RNA, Messenger, analysis, metabolism, Receptors, Thyrotropin, biosynthesis, Thyroid Gland, cytology, metabolism, Thyroid Neoplasms, metabolism, pathology
Ämneskategorier Endokrinologi

Sammanfattning

Loss of E-cadherin (uvomorulin), a Ca(2+)-dependent cell adhesion molecule required for normal epithelial function, has been attributed a pathogenetic role in tumor invasion. The expression of E-cadherin was studied in normal and neoplastic follicular epithelium of the human thyroid by Northern blot analysis and immunofluorescence on frozen tissue sections. In the normal thyroid (n = 10) and in benign thyroid disorders (n = 21; toxic diffuse goitre; multinodular goitre; follicular adenomas), E-cadherin mRNA levels were equally high and the follicles were generally stained, mainly along the lateral surface of the epithelial cells, by the anti-E-cadherin monoclonal antibody. In anaplastic thyroid carcinomas (n = 6) E-cadherin expression was very low or lacking. In papillary carcinomas (n = 23), E-cadherin mRNA levels varied from nearly normal to highly reduced, which roughly correlated with the overall immunofluorescence intensity. However, the immunostaining also revealed a heterogeneous "all-or-nothing" expression of E-cadherin among adjacent cells in the same tumor. In the follicular carcinomas (n = 9), E-cadherin mRNA levels were in general rather high but the immunostaining varied considerably. A few papillary and follicular tumors lacked immunoreactive E-cadherin in spite of high mRNA levels. In oxyphilic (Hürthle) cell tumors, comprising both adenomas (n = 4) and carcinomas (n = 2), E-cadherin immunoreactivity was reduced and distributed intracellularly rather than at the cell surface. The expression of E-cadherin in relapsing thyroid carcinomas and in tumors with metastatic spreading was, irrespective of the histiotype, low or lacking. Sequential Northern analysis revealed a close correlation between the expression levels of E-cadherin and the thyrotropin receptor. Together, the data suggest that in human thyroid malignancies both gene expression and posttranscriptional control of E-cadherin may be impaired.

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