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No difference in human mast cells derived from peanut allergic versus non-allergic subjects

Artikel i vetenskaplig tidskrift
Författare L. F. Larsen
N. Juel-Berg
A. Hansen
K. S. Hansen
E. N. C. Mills
R. van Ree
Madeleine Rådinger
L. K. Poulsen
B. M. Jensen
Publicerad i Immunity Inflammation and Disease
Volym 6
Nummer/häfte 4
Sidor 416-427
ISSN 2050-4527
Publiceringsår 2018
Publicerad vid Krefting Research Centre
Institutionen för medicin, avdelningen för invärtesmedicin och klinisk nutrition
Sidor 416-427
Språk en
Länkar dx.doi.org/10.1002/iid3.226
Ämnesord Food allergy, mast cells, peanut, fc-epsilon-ri, ige-mediated activation, receptor cross-linking, necrosis-factor-alpha, peripheral-blood, serum ige, t-cells, release, murine, heterogeneity, Immunology
Ämneskategorier Lungmedicin och allergi

Sammanfattning

Introduction Mast cells are the primary effector cells of allergy. This study aimed at characterizing human peripheral blood-derived mast cells (PBdMC) from peanut allergic and non-allergic subjects by investigating whether the molecular and stimulus-response profile of PBdMC discriminate between peanut allergic and healthy individuals. Methods Results PBdMC were generated from eight peanut allergic and 10 non-allergic subjects. The molecular profile (cell surface receptor expression) was assessed using flow cytometry. The stimulus-response profile (histamine release induced by secretagogues, secretion of cytokines/chemokines and changes in miRNA expression following anti-IgE activation) was carried out with histamine release test, luminex multiplex assay and miRNA arrays. Expression of activating receptors (Fc epsilon RI, CD48, CD88, CD117, and C3aR) on PBdMC was not different among peanut allergic and non-allergic subjects. Likewise, inhibitory receptors (CD32, CD200R, CD300a, and siglec-8) displayed comparable levels of expression. Both groups of PBdMC were unresponsive to substance P, compound 48/80 and C5a but released comparable levels of histamine when stimulated with anti-IgE and C3a. Interestingly, among the secreted cytokines/chemokines (IL-8, IL-10, IL-13, IL-23, IL-31, IL-37, MCP-1, VEGF, GM-CSF) PBdMC from peanut allergic subjects showed a different secretion pattern of IL-31 compared to non-allergic subjects. Investigating miRNA expression from resting or activated PBdMC revealed no significantly difference between peanut allergic and non-allergic subjects. Conclusion The molecular and stimulus-response profile revealed that PBdMC from peanut allergic subjects differently express IL-31 compared to non-allergic subjects. However, since only one altered parameter was found among 893 investigated, it is still questionable if the pathophysiological mechanisms of peanut allergy are revealed in PBdMC.

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