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Molecular microheterogeneity of prostate specific antigen in seminal fluid by mass spectrometry

Artikel i vetenskaplig tidskrift
Författare Ákos Végvári
Melinda Rezeli
Carina Sihlbom
Jari Häkkinen
Elisabet Carlsohn
Johan Malm
Hans Lilja
Thomas Laurell
György Marko-Varga
Publicerad i Clinical Biochemistry
Volym 45
Nummer/häfte 4-5
Sidor 331-338
ISSN 00099120
Publiceringsår 2012
Publicerad vid Core Facilities, Proteomics
Sidor 331-338
Språk en
Länkar dx.doi.org/10.1016/j.clinbiochem.20...
Ämnesord ESI-LTQ FT-ICR, Infertility, Isoform, MALDI LTQ Orbitrap XL, Prostate specific antigen, Seminal plasma
Ämneskategorier Biokemi

Sammanfattning

Objectives: Prostate specific antigen (PSA) is a widely used and clinically valuable marker for prostate disease. In order to enable the development of new PSA assays and progress the understanding of the biology of PSA we have analyzed PSA in seminal plasma.Design and methods. PSA in seminal plasma from men attending a fertility clinic and healthy controls was analyzed using SDS-PAGE, Western blotting and mass spectrometry. Results: Using mass spectrometry, different forms of PSA could be identified in 1-9 bands seen on SDS-PAGE analysis of the respective sample. However, a majority of these molecular forms of PSA were not observed on Western blots. Enzymatic activity of PSA isoforms was demonstrated by sequencing data in zymogram gels. Multivariate analysis of clinical data revealed well-separated patient groups. Conclusions: We demonstrated that PSA in seminal plasma occurs in several isoforms, yet not all were detectable using an antibody based clinical routine method. The heterogeneity of PSA expression might be of clinical significance, by an improved patient phenotyping. © 2011 Elsevier B.V.

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