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Electrochemical Investigation of the Interaction between Catecholamines and ATP

Artikel i vetenskaplig tidskrift
Författare Z. Taleat
J. Estevez-Herrera
J. D. Machado
J. Dunevall
Andrew G Ewing
R. Borges
Publicerad i Analytical Chemistry
Volym 90
Nummer/häfte 3
Sidor 1601-1607
ISSN 0003-2700
Publiceringsår 2018
Publicerad vid Institutionen för kemi och molekylärbiologi
Sidor 1601-1607
Språk en
Länkar dx.doi.org/10.1021/acs.analchem.7b0...
Ämnesord adrenal chromaffin granules, nuclear-magnetic-resonance, single-cell, amperometry, divalent metal-ions, diffusion-coefficients, chromogranin-a, exocytosis, fusion, vesicles, release, Chemistry, emical microenvironment
Ämneskategorier Molekylärbiologi

Sammanfattning

The study of the colligative properties of adenosine 5'-triphosphate (ATP) and catecholamines has received the attention of scientists for decades, as they could explain the capabilities of secretory vesicles (SVs) to accumulate neurotransmitters. In this Article, we have applied electrochemical methods to detect such interactions in vitro, at the acidic pH of SVs (pH 5.5) and examined the effect of compounds having structural similarities that correlate with functional groups of ATP (adenosine, phosphoric acid and sodium phosphate salts) and catecholamines (catechol). Chronoamperometry and fast scan cyclic voltammetry (FSCV) provide evidence compatible with an interaction of the catechol and adenine rings. This interaction is also reinforced by an electrostatic interaction between the phosphate group of ATP and the protonated ammonium group of catecholamines. Furthermore, chronoamperometry data suggest that the presence of ATP subtlety reduces the apparent diffusion coefficient of epinephrine in aqueous media that adds an additional factor leading to a slower rate of catecholamine exocytosis. This adds another plausible mechanism to regulate individual exocytosis events to alter communication.

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