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Sample handling of gastric tissue and O-glycan alterations in paired gastric cancer and non-tumorigenic tissues

Artikel i vetenskaplig tidskrift
Författare Barbara Adamczyk
Chunsheng Jin
K. Polom
P. Munoz
Miguel A. Rojas-Macias
D. Zeeberg
M. Boren
F. Roviello
Niclas G. Karlsson
Publicerad i Scientific Reports
Volym 8
ISSN 2045-2322
Publiceringsår 2018
Publicerad vid Institutionen för biomedicin, avdelningen för medicinsk kemi och cellbiologi
Språk en
Länkar doi.org/10.1038/s41598-017-18299-6
Ämnesord mass-spectrometry, glycoprofiling platform, linked oligosaccharides, glycosylation analysis, unicarb-db, extraction, stabilization, glycoproteins, nomenclature, biomarker
Ämneskategorier Medicinsk bioteknologi (med inriktning mot cellbiologi (inklusive stamcellsbiologi), molekylärbiologi, mikrobiologi, biokemi eller biofarmaci), Cancer och onkologi

Sammanfattning

Sample collection, handling and storage are the most critical steps for ensuring the highest preservation of specimens. Pre-analytical variability can influence the results as protein signatures alter rapidly after tissue excision or during long-term storage. Hence, we evaluated current state-of-the-art biobank preservation methods from a glycomics perspective and analyzed O-glycan alterations occurring in the gastric cancer tissues. Paired tumor and adjacent normal tissue samples were obtained from six patients undergoing gastric cancer surgery. Collected samples (n = 24) were either snap-frozen or heat stabilized and then homogenized. Glycans were released from extracted glycoproteins and analyzed by LC-MS/MS. In total, the relative abundance of 83 O-glycans and 17 derived structural features were used for comparison. There was no statistically significant difference found in variables between snap frozen and heat-stabilized samples, which indicated the two preservation methods were comparable. The data also showed significant changes between normal and cancerous tissue. In addition to a shift from high sialylation in the cancer area towards blood group ABO in the normal area, we also detected that the LacdiNAc epitope (N, N'-diacetyllactosamine) was significantly decreased in cancer samples. The O-glycan alterations that are presented here may provide predictive power for the detection and prognosis of gastric cancer.

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