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Cold-perfusion decellularization of whole-organ porcine pancreas supports human fetal pancreatic cell attachment and expression of endocrine and exocrine markers

Artikel i vetenskaplig tidskrift
Författare Erik Elebring
Vijay Kumar Kuna
Niclas Kvarnström
Suchitra Sumitran-Holgersson
Publicerad i Journal of Tissue Engineering
Volym 8
Publiceringsår 2017
Publicerad vid Institutionen för kliniska vetenskaper, Avdelningen för kirurgi
Språk en
Länkar doi.org/10.1177/2041731417738145
Ämnesord Pancreas, decellularization, cold-perfusion, recellularization, human fetal pancreatic stem cells, insulin, glucagon, amylase
Ämneskategorier Kirurgi

Sammanfattning

Despite progress in the field of decellularization and recellularization, the outcome for pancreas has not been adequate. This might be due to the challenging dual nature of pancreas with both endocrine and exocrine tissues. We aimed to develop a novel and efficient cold-perfusion method for decellularization of porcine pancreas and recellularize acellular scaffolds with human fetal pancreatic stem cells. Decellularization of whole porcine pancreas at 4 degrees C with sodium deoxycholate, Triton X-100 and DNase efficiently removed cellular material, while preserving the extracellular matrix structure. Furthermore, recellularization of acellular pieces with human fetal pancreatic stem cells for 14 days showed attached and proliferating cells. Both endocrine (C-peptide and PDX1) and exocrine (glucagon and -amylase) markers were expressed in recellularized tissues. Thus, cold-perfusion can successfully decellularize porcine pancreas, which when recellularized with human fetal pancreatic stem cells shows relevant endocrine and exocrine phenotypes. Decellularized pancreas is a promising biomaterial and might translate to clinical relevance for treatment of diabetes.

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