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Site-specific identification of heparan and chondroitin sulfate glycosaminoglycans in hybrid proteoglycans

Artikel i vetenskaplig tidskrift
Författare Fredrik Noborn
Alejandro Gomez Toledo
Anders Green
Waqas Nasir
Carina Sihlbom
Jonas Nilsson
Göran Larson
Publicerad i Scientific Reports
Volym 6
Publiceringsår 2016
Publicerad vid Institutionen för biomedicin, avdelningen för klinisk kemi och transfusionsmedicin
Core Facilities, Proteomics
Språk en
Länkar dx.doi.org/10.1038/srep34537
Ämneskategorier Klinisk laboratoriemedicin

Sammanfattning

© 2016 The Author(s).Heparan sulfate (HS) and chondroitin sulfate (CS) are complex polysaccharides that regulate important biological pathways in virtually all metazoan organisms. The polysaccharides often display opposite effects on cell functions with HS and CS structural motifs presenting unique binding sites for specific ligands. Still, the mechanisms by which glycan biosynthesis generates complex HS and CS polysaccharides required for the regulation of mammalian physiology remain elusive. Here we present a glycoproteomic approach that identifies and differentiates between HS and CS attachment sites and provides identity to the core proteins. Glycopeptides were prepared from perlecan, a complex proteoglycan known to be substituted with both HS and CS chains, further digested with heparinase or chondroitinase ABC to reduce the HS and CS chain lengths respectively, and thereafter analyzed by nLC-MS/MS. This protocol enabled the identification of three consensus HS sites and one hybrid site, carrying either a HS or a CS chain. Inspection of the amino acid sequence at the hybrid attachment locus indicates that certain peptide motifs may encode for the chain type selection process. This analytical approach will become useful when addressing fundamental questions in basic biology specifically in elucidating the functional roles of site-specific glycosylations of proteoglycans.

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