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An inflammatory equine model demonstrates dynamic changes of immune response and cartilage matrix molecule degradation in vitro

Artikel i vetenskaplig tidskrift
Författare Emilia Svala
M. Lofgren
Carina Sihlbom
Ulla Rüetschi
Anders Lindahl
S. Ekman
Eva Skiöldebrand
Publicerad i Connective Tissue Research
Volym 56
Nummer/häfte 4
Sidor 315-325
ISSN 0300-8207
Publiceringsår 2015
Publicerad vid Institutionen för biomedicin, avdelningen för klinisk kemi och transfusionsmedicin
Core Facilities, Proteomics
Sidor 315-325
Språk en
Länkar dx.doi.org/10.3109/03008207.2015.10...
Ämnesord Horse, IL-1 beta, matrix molecules, osteoarthritis, proteomics, HUMAN SYNOVIAL-FLUID, ARTICULAR-CARTILAGE, JOINT DISEASE, PROTEIN, THROMBOSPONDIN-5, INTERGLOBULAR DOMAIN, AGGRECAN FRAGMENTS, COMPLEMENT-SYSTEM, COLLAGEN-IX, OSTEOARTHRITIS, INTERLEUKIN-1-BETA
Ämneskategorier Klinisk medicin

Sammanfattning

The molecular aspects of inflammation were investigated in equine articular cartilage explants using quantitative proteomics. Articular cartilage explants were stimulated with interleukin (IL)-1 beta in vitro for 25 days, and proteins released into cell culture media were chemically labeled with isobaric mass tags and analyzed by liquid chromatography-tandem mass spectrometry. A total of 127 proteins were identified and quantified in media from explants. IL-1 beta-stimulation resulted in an abundance of proteins related to inflammation, including matrix metalloproteinases, acute phase proteins, complement components and IL-6. Extracellular matrix (ECM) molecules were released at different time points, and fragmentation of aggrecan and cartilage oligomeric matrix protein was observed at days 3 and 6, similar to early-stage OA in vivo. Degradation products of the collagenous network were observed at days 18 and 22, similar to late-stage OA. This model displays a longitudinal quantification of released molecules from the ECM of articular cartilage. Identification of dynamic changes of extracellular matrix molecules in the secretome of equine explants stimulated with IL-1 beta over time may be useful for identifying components released at different time points during the spontaneous OA process.

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