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The human gut microbiome as a transporter of antibiotic resistance genes between continents

Artikel i vetenskaplig tidskrift
Författare Johan Bengtsson-Palme
Martin Angelin
Mikael Huss
Sanela Kjellqvist
Erik Kristiansson
Helena Palmgren
D. G. Joakim Larsson
Anders Johansson
Publicerad i Antimicrobial Agents and Chemotherapy
Volym 59
Nummer/häfte 10
Sidor 6551-6560
ISSN 0066-4804
Publiceringsår 2015
Publicerad vid Institutionen för matematiska vetenskaper, matematisk statistik
Institutionen för biomedicin, avdelningen för infektionssjukdomar
Sidor 6551-6560
Språk en
Länkar dx.doi.org/10.1128/AAC.00933-15
Ämneskategorier Bioinformatik (beräkningsbiologi), Mikrobiologi, Mikrobiologi

Sammanfattning

Previous studies of antibiotic resistance dissemination by travel have, by targeting only a select number of cultivable bacterial species, omitted most of the human microbiome. Here, we used explorative shotgun metagenomic sequencing to address the abundance of >300 antibiotic resistance genes in fecal specimens from 35 Swedish students taken before and after exchange programs on the Indian peninsula or in Central Africa. All specimens were additionally cultured for extended-spectrum beta-lactamase (ESBL)-producing enterobacteria, and the isolates obtained were genome sequenced. The overall taxonomic diversity and composition of the gut microbiome remained stable before and after travel, but there was an increasing abundance of Proteobacteria in 25/35 students. The relative abundance of antibiotic resistance genes increased, most prominently for genes encoding resistance to sulfonamide (2.6-fold increase), trimethoprim (7.7-fold), and beta-lactams (2.6-fold). Importantly, the increase observed occurred without any antibiotic intake. Of 18 students visiting the Indian peninsula, 12 acquired ESBL-producing Escherichia coli, while none returning from Africa were positive. Despite deep sequencing efforts, the sensitivity of metagenomics was not sufficient to detect acquisition of the low-abundant genes responsible for the observed ESBL phenotype. In conclusion, metagenomic sequencing of the intestinal microbiome of Swedish students returning from exchange programs in Central Africa or the Indian peninsula showed increased abundance of genes encoding resistance to widely used antibiotics.

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