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Structural characterization of non-acid glycosphingolipids in kidneys of single blood group O and A pigs.

Artikel i vetenskaplig tidskrift
Författare Jan Holgersson
Per-Åke Jovall
Bo Samuelsson
Michael Breimer
Publicerad i Journal of biochemistry
Volym 108
Nummer/häfte 5
Sidor 766-77
ISSN 0021-924X
Publiceringsår 1990
Publicerad vid Medicinska institutionen
Sidor 766-77
Språk en
Länkar www.ncbi.nlm.nih.gov/entrez/query.f...
Ämnesord ABO Blood-Group System, immunology, Animals, Blood Transfusion, Carbohydrate Sequence, Chromatography, High Pressure Liquid, Chromatography, Thin Layer, Glycosphingolipids, chemistry, immunology, Kidney, chemistry, immunology, Magnetic Resonance Spectroscopy, Mass Spectrometry, Models, Biological, Molecular Sequence Data, Swine
Ämneskategorier Klinisk medicin

Sammanfattning

Total non-acid glycosphingolipids were isolated from the kidneys of single pigs serologically typed on their red blood cells as blood groups O and A. Glycolipid species were purified by HPLC and structurally characterized by thin-layer chromatography, mass spectrometry, proton NMR spectroscopy, degradation analysis, and reactivity with various monoclonal antibodies, Gal alpha 1-4Gal-specific E. coli bacteria, and lectins. Glucosyl-, globotriaosyl-, and globotetraosylceramides were the predominant molecular species with lactosyl- and globopentaosylceramides (IV3GalGb4Cer) as abundant constituents too. Small amounts of galactosyl- and digalactosylceramides were also present. In the blood group O pig kidneys, blood group H antigens based on four different core saccharides (types 1, 2, 4, and lactosyl core) were identified and the major blood group structure was V2FucIV3Gal-Gb4Cer. In the kidneys from the blood group A pig the corresponding blood group A antigens were found and in addition, a type 3 chain blood group A antigen was indicated by mass spectrometry and by its reactivity with a monoclonal antibody. Trace amounts of the type 2 chain-based X and Y antigens were found while blood group B antigens and the type 1 chain based Lewis antigens could not be detected. The ceramide part of the glycolipids was mainly composed of dihydroxy 18:0 long chain bases and non-hydroxy 16:0-24:0 fatty cids.

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