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Characterisation of human natural anti-sheep xenoantibodies.

Artikel i vetenskaplig tidskrift
Författare V Strokan
Lennart Rydberg
Eva C. Hallberg
Johan Mölne
Michael Breimer
Publicerad i Xenotransplantation
Volym 5
Nummer/häfte 2
Sidor 111-21
ISSN 0908-665X
Publiceringsår 1998
Publicerad vid Institutionen för laboratoriemedicin , Avdelningen för patologi
Institutionen för laboratoriemedicin, Avdelningen för klinisk kemi/transfusionsmedicin
Institutionen för de kirurgiska disciplinerna, Avdelningen för kirurgi
Sidor 111-21
Språk en
Länkar www.ncbi.nlm.nih.gov/entrez/query.f...
Ämnesord Animals, Antibodies, Heterophile, blood, isolation & purification, Antigens, Heterophile, chemistry, metabolism, Antilymphocyte Serum, blood, Carbohydrate Sequence, Forssman Antigen, metabolism, Glycolipids, chemistry, immunology, Hemagglutinins, blood, Humans, Immunity, Innate, Immunohistochemistry, Immunosorbent Techniques, Kidney, immunology, Kidney Transplantation, adverse effects, immunology, Molecular Sequence Data, Perfusion, Sheep, immunology, Swine, Transplantation, Heterologous, adverse effects
Ämneskategorier Transplantationskirurgi

Sammanfattning

Currently, the pig species is regarded as the most likely organ donor for human xenotransplantation in the future. However, it cannot be granted that the pig will be the optimal species of choice. We have studied human anti-sheep antibodies in comparison with anti-pig antibodies. The anti-sheep lymphocytotoxic and hemagglutination titers were in the range 8 to 128 and 2 to 32, respectively, in single individuals, which were considerably lower than the anti-pig titers of these individuals. Perfusion of sheep kidneys with human blood reduced the anti-sheep xenoantibody titers to zero as measured by lymphocytotoxic, hemagglutination, and sheep aortic endothelial cell antibody binding assays. The perfused kidneys showed generalised depositions of human IgM and C3c in the vascular tree and focal depositions of C1q and fibrin. Obliteration of capillaries by human platelets and polymorphonuclear cells were observed. Total neutral glycolipid fractions were isolated from sheep intestinal, pancreatic, and kidney tissues. By using a chromatogram binding assay, a monoclonal anti-Forssman antibody identified a single compound with five sugar residues in all organs. Several glycolipid bands were stained in all organs by the Gal(alpha)1-specific lectin I-B4 from Griffonia (Bandeiraea) Simplicifolia. A human AB serum pool showed staining by both IgG and IgM antibodies of the Forssman and Gal(alpha)1-terminating components as well as some other, not structurally identified, components. The Forssman and Gal(alpha)1-reactivity in human sera could be eliminated by immunoadsorption using Forssman and Gal(alpha)1-3Gal-immunoadsorbent columns, respectively. Immunostaining of sheep kidney tissue sections showed the presence of Gal(alpha)1-terminating epitopes by immunoperoxidase and immunogold silver staining techniques. Proximal convoluted tubules showed a strong staining, while thin loops of Henle, collecting ducts, urothelium, and vessels showed a weaker staining. Distal convoluted tubules and thick loops of Henle were completely negative. In summary, human serum contains anti-sheep xenoantibodies reacting mainly with the Forssman and Gal(alpha)1-determinants in sheep tissues and the anti-sheep antibody titers are lower than the corresponding anti-pig titers.

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