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Studies of mucus in mouse stomach, small intestine, and colon. II. Gastrointestinal mucus proteome reveals Muc2 and Muc5ac accompanied by a set of core proteins.

Artikel i vetenskaplig tidskrift
Författare Ana María Rodríguez-Piñeiro
Joakim H. Bergström
Anna Ermund
Jenny K Gustafsson
André Schütte
Malin E V Johansson
Gunnar C. Hansson
Publicerad i American journal of physiology. Gastrointestinal and liver physiology
Volym 305
Nummer/häfte 5
Sidor G348-56
ISSN 1522-1547
Publiceringsår 2013
Publicerad vid Institutionen för biomedicin, avdelningen för medicinsk kemi och cellbiologi
Sidor G348-56
Språk en
Länkar dx.doi.org/10.1152/ajpgi.00047.2013
Ämnesord Animals, Biotinylation, Chromatography, High Pressure Liquid, Chromatography, Reverse-Phase, Cluster Analysis, Colon, metabolism, Female, Gastric Mucosa, metabolism, Intestinal Mucosa, metabolism, Intestine, Small, metabolism, Male, Mice, Mice, Inbred C57BL, Mucin 5AC, metabolism, Mucin-2, metabolism, Mucus, metabolism, Proteomics, methods, Spectrometry, Mass, Electrospray Ionization, Tandem Mass Spectrometry
Ämneskategorier Cell- och molekylärbiologi

Sammanfattning

The mucus that protects the surface of the gastrointestinal tract is rich in specialized O-glycoproteins called mucins, but little is known about other mucus proteins or their variability along the gastrointestinal tract. To ensure that only mucus was analyzed, we combined collection from explant tissues mounted in perfusion chambers, liquid sample preparation, single-shot mass spectrometry, and specific bioinformatics tools, to characterize the proteome of the murine mucus from stomach to distal colon. With our approach, we identified ∼1,300 proteins in the mucus. We found no differences in the protein composition or abundance between sexes, but there were clear differences in mucus along the tract. Noticeably, mucus from duodenum showed similarities to the stomach, probably reflecting the normal distal transport. Qualitatively, there were, however, fewer differences than might had been anticipated, suggesting a relatively stable core proteome (∼80% of the total proteins identified). Quantitatively, we found significant differences (∼40% of the proteins) that could reflect mucus specialization throughout the gastrointestinal tract. Hierarchical clustering pinpointed a number of such proteins that correlated with Muc2 (e.g., Clca1, Zg16, Klk1). This study provides a deeper knowledge of the gastrointestinal mucus proteome that will be important in further understanding this poorly studied mucosal protection system.

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