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Ultrastructural evaluation of shrinkage artefacts induced by fixatives and embedding resins on osteocyte processes and pericellular space dimensions

Artikel i vetenskaplig tidskrift
Författare Furqan A. Shah
Bengt R Johansson
Peter Thomsen
Anders Palmquist
Publicerad i Journal of Biomedical Materials Research. Part A
Volym 103
Nummer/häfte 4
Sidor 1565-76
ISSN 1549-3296
Publiceringsår 2015
Publicerad vid Institutionen för kliniska vetenskaper, Avdelningen för biomaterialvetenskap
Institutionen för biomedicin, avdelningen för medicinsk kemi och cellbiologi
Sidor 1565-76
Språk en
Länkar dx.doi.org/10.1002/jbm.a.35287
Ämneskategorier Strukturbiologi, Cellbiologi, Medicinska material och protesteknik, Biomaterialvetenskap

Sammanfattning

The integrity of the interface between the osteocyte (Ot) process and the canalicular wall was investigated in terms of change in the lateral dimensions of the Ot process in relation to the canalicular width, i.e., widening of the pericellular space. This has been interpreted as shrinkage of the Ot process relative to the canalicular wall during sample preparation stages of fixation, dehydration, and resin embedding. Sprague-Dawley rat tibial cross-sections were prepared for transmission electron microscopy (TEM). Four different fixative preparations: paraformaldehyde (PF), modified Karnovsky's (MK), glutaraldehyde (GRR) with ruthenium red (GRR), and zinc formalin (ZF); and two different embedding resins: LR Gold (LRG) and Epon812 (Epon) were evaluated. It was found that for LRG embedding, formalin-only fixatives (PF and ZF) induced lower shrinkage than GRR-containing fixatives (MK and GRR). In contrast, for Epon embedding, MK showed the highest shrinkage, while no differences were found between the remaining fixatives (PF, ZF, and GRR). All formalin-containing fixatives (MK, PF, and ZF) induced similar shrinkage in both embedding media. The most dramatic difference was for GRR fixation, which in combination with LRG embedding showed ∼62% more shrinkage than with Epon embedding, suggesting that the combination of GRR fixation and LRG embedding synergistically amplifies Ot shrinkage. These differences likely suggest a role of the resin in secondarily influencing the tissue structure following fixation. Further, the work confirms LRG as a poor embedding medium for bone specimens, as it causes large variations in shrinkage depending on fixation. © 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2014.

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