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A new approach to measuring vitamin D in human adipose tissue using time-of-flight secondary ion mass spectrometry: A pilot study

Artikel i vetenskaplig tidskrift
Författare Per Malmberg
Therese Karlsson
Henrik Svensson
Malin Lönn
Nils-Gunnar Carlsson
Ann-Sofie Sandberg
Eva Jennische
Amra Osmancevic
Agneta Holmäng
Publicerad i Journal of Photochemistry and Photobiology. B: Biology
Volym 138
Sidor 295-301
ISSN 1011-1344
Publiceringsår 2014
Publicerad vid Institutionen för neurovetenskap och fysiologi
Institutionen för neurovetenskap och fysiologi, sektionen för fysiologi
Institutionen för biomedicin, avdelningen för klinisk kemi och transfusionsmedicin
Sidor 295-301
Språk en
Länkar dx.doi.org/10.1016/j.jphotobiol.201...
Ämnesord Adipose tissue, Vitamin D, TOF-SIMS
Ämneskategorier Dermatologi och venereologi

Sammanfattning

Circulating concentrations of vitamin D, 25(OH)D, and 1,25(OH)2D are lower in obese than lean individuals, but little is known about the adipose tissue content of these molecules. The aim of this study was to explore the possibility to use time-of-flight secondary ion mass spectrometry (TOF-SIMS) to measure vitamin D and its metabolites in fat tissue in obese and lean subjects. Abdominal subcutaneous adipose tissue (SAT) biopsies were obtained from three lean and three obese women, and paired biopsies SAT and visceral adipose tissue (VAT) were obtained from three obese subjects during gastric bypass surgery. TOF-SIMS was used to measure vitamin D3, 25(OH)D3, and 1,25(OH)2D3 in adipose tissue. We found that vitamin D3, 25(OH)D3, and 1,25(OH)2D3 in adipose tissue can be measured with TOF-SIMS. In adipose tissue, vitamin D3 and its metabolites were located in adipocyte lipid droplets. The content of vitamin D3 (P = 0.006) and 25(OH)D3 (P = 0.018) were lower in SAT in obese compared with lean women. TOF-SIMS has the potential to semi-quantitatively measure vitamin D metabolites in adipose tissue, and offers a possibility to compare vitamin D levels in different depots and groups of individuals. It also gives the opportunity to explore the localization of vitamin D metabolites at a cellular level.

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