Till sidans topp

Sidansvarig: Webbredaktion
Sidan uppdaterades: 2012-09-11 15:12

Tipsa en vän
Utskriftsversion

In vitro and in vivo beha… - Göteborgs universitet Till startsida
Webbkarta
Till innehåll Läs mer om hur kakor används på gu.se

In vitro and in vivo behavior of 111In-labelled platelets: an experimental study of healthy male volunteers.

Artikel i vetenskaplig tidskrift
Författare Hans Wadenvik
Stefan Jacobsson
Jack Kutti
M Syrjälä
Publicerad i European journal of haematology
Volym 38
Nummer/häfte 5
Sidor 415-25
ISSN 0902-4441
Publiceringsår 1987
Publicerad vid Medicinska institutionen
Sidor 415-25
Språk en
Länkar www.ncbi.nlm.nih.gov/entrez/query.f...
Ämnesord Adult, Blood Platelets, physiology, Cell Survival, Evaluation Studies as Topic, Humans, Indium Radioisotopes, diagnostic use, Male, Middle Aged, Platelet Aggregation, Platelet Transfusion, Spleen, blood supply, metabolism
Ämneskategorier Klinisk medicin

Sammanfattning

The aim of this study was to obtain a critical evaluation of a simple method for labelling platelets with 111In-oxine. All experiments were carried out on healthy volunteers. 65 +/- 7 (SD) % of the platelets in collected blood were labelled and reinjected. As compared to control experiments, only in response to a low final ADP concentration (1.0 mumol/l) did 111In-labelled platelets show reduced in vitro aggregability. The mean platelet volume for 111In-labelled platelets was slightly lower than the mean platelet volume in whole blood. The results for initial platelet recovery and platelet mean lifespan closely agreed with those of other studies in which considerably higher platelet extraction from whole blood was obtained. After injection, the splenic uptake and blood disappearance of 111In-labelled platelets followed a monoexponential function with almost identical rate constants. By compartmental analysis of the equilibration of platelets between blood and spleen, the splenic blood flow was estimated to be 4.8 +/- 1.9 (SD) % of the total blood volume/min; the intrasplenic platelet transit time was 9.7 +/- 1.6 (SD) min, and the exchangeable splenic platelet pool 31 +/- 8 (SD) %. Highly significant relationships were present between the splenic blood flow and the splenic platelet pool size, as well as between the splenic blood flow and the initial platelet recovery. It is concluded that the requirements for adequate interpretation of platelet kinetics are well met with the present method for harvesting and labelling of platelets.

Sidansvarig: Webbredaktion|Sidan uppdaterades: 2012-09-11
Dela:

På Göteborgs universitet använder vi kakor (cookies) för att webbplatsen ska fungera på ett bra sätt för dig. Genom att surfa vidare godkänner du att vi använder kakor.  Vad är kakor?