Till sidans topp

Sidansvarig: Webbredaktion
Sidan uppdaterades: 2012-09-11 15:12

Tipsa en vän
Utskriftsversion

Quantitative proteomics r… - Göteborgs universitet Till startsida
Webbkarta
Till innehåll Läs mer om hur kakor används på gu.se

Quantitative proteomics reveals regulatory differences in the chondrocyte secretome from human medial and lateral femoral condyles in osteoarthritic patients

Artikel i vetenskaplig tidskrift
Författare Johan Stenberg
Ulla Rüetschi
Eva Skiöldebrand
Johan Kärrholm
Anders Lindahl
Publicerad i Proteome Science
Volym 11
Nummer/häfte 1
Sidor UNSP 43
ISSN 1477-5956
Publiceringsår 2013
Publicerad vid Institutionen för biomedicin, avdelningen för klinisk kemi och transfusionsmedicin
Institutionen för kliniska vetenskaper, Avdelningen för ortopedi
Sidor UNSP 43
Språk en
Länkar dx.doi.org/10.1186/1477-5956-11-43
Ämnesord Secretome, SILAC, Chondrocyte, Osteoarthritis, Proteomics, HUMAN ARTICULAR CHONDROCYTES, ENDOTHELIAL-CELL MIGRATION, PROTEIN, PHOSPHATASE 2A, LARGE GENE LISTS, SIGNALING PATHWAY, KNEE, OSTEOARTHRITIS, BIOCHEMICAL MARKER, SYNOVIAL-FLUIDS, CARTILAGE, IDENTIFICATION
Ämneskategorier Ortopedi, Cell- och molekylärbiologi

Sammanfattning

Background: Osteoarthritis (OA) is a destructive joint disease and there are no known biomarkers available for an early diagnosis. To identify potential disease biomarkers and gain further insight into the disease mechanisms of OA we applied quantitative proteomics with SILAC technology on the secretomes from chondrocytes of OA knees, designated as high Mankin (HM) scored secretome. A quantitative comparison was made between the secretomes of the medial and lateral femur condyle chondrocytes in the same knee since the medial femur condyle is usually more affected in OA than the lateral condyle, which was confirmed by Mankin scoring. The medial/lateral comparison was also made on the secretomes from chondrocytes taken from one individual with no clinically apparent joint-disease, designated as low Mankin (LM) scored secretome. Results: We identified 825 proteins in the HM secretome and 69 of these showed differential expression when comparing the medial and lateral femoral compartment. The LM scored femoral condyle showed early signs of OA in the medial compartment as assessed by Mankin score. We here report the identification and relative quantification of several proteins of interest for the OA disease mechanism e.g. CYTL1, DMD and STAB1 together with putative early disease markers e.g. TIMP1, PPP2CA and B2M. Conclusions: The present study reveals differences in protein abundance between medial/lateral femur condyles in OA patients. These regulatory differences expand the knowledge regarding OA disease markers and mechanisms.

Sidansvarig: Webbredaktion|Sidan uppdaterades: 2012-09-11
Dela:

På Göteborgs universitet använder vi kakor (cookies) för att webbplatsen ska fungera på ett bra sätt för dig. Genom att surfa vidare godkänner du att vi använder kakor.  Vad är kakor?