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Vitamin D enhances IL-1β secretion and restricts growth of Mycobacterium tuberculosis in macrophages from TB patients

Artikel i vetenskaplig tidskrift
Författare Daniel Eklund
Hans Lennart Persson
Marie Larsson
Amanda Welin
Jonna Idh
Jakob Paues
Sven-Göran Fransson
Olle Stendahl
Thomas Schön
Maria Lerm
Publicerad i International Journal of Mycobacteriology
Volym 2
Nummer/häfte 1
Sidor 18-25
ISSN 2212-5531
Publiceringsår 2013
Publicerad vid Institutionen för medicin, avdelningen för reumatologi och inflammationsforskning
Sidor 18-25
Språk en
Länkar dx.doi.org/10.1016/j.ijmyco.2012.11...
Ämnesord Vitamin D, Human macrophages, Intracellular growth, TB patients, IL-1β
Ämneskategorier Immunbiologi, Medicinsk mikrobiologi

Sammanfattning

The emergence of multidrug-resistant strains of Mycobacterium tuberculosis (MTB), the bacterium responsible for tuberculosis (TB), has rekindled the interest in the role of nutritional supplementation of micronutrients, such as vitamin D, as adjuvant treatment. Here, the growth of virulent MTB in macrophages obtained from the peripheral blood of patients with and without TB was studied. The H37Rv strain genetically modified to express Vibrio harveyi luciferase was used to determine the growth of MTB by luminometry in the human monocyte-derived macrophages (hMDMs) from study subjects. Determination of cytokine levels in culture supernatants was performed using a flow cytometry-based bead array technique. No differences in intracellular growth of MTB were observed between the different study groups. However, stimulation with 100 nM 1,25-dihydroxyvitamin D significantly enhanced the capacity of hMDMs isolated from TB patients to control the infection. This effect was not observed in hMDMs from the other groups. The interleukin (IL)-1β and IL-10 release by hMDMs was clearly increased upon stimulation with 1,25-dihydroxyvitamin D. Furthermore, the 1,25-dihydroxyvitamin D stimulation also led to elevated levels of TNF-α (tumor necrosis factor-alpha) and IL-12p40. It was concluded that vitamin D triggers an inflammatory response in human macrophages with enhanced secretion of cytokines, as well as enhancing the capacity of hMDMs from patients with active TB to restrict mycobacterial growth.

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