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Left atrium of the human adult heart contains a population of side population cells.

Artikel i vetenskaplig tidskrift
Författare Joakim Sandstedt
Marianne Jonsson
Kristina Kajic
Mikael Sandstedt
Anders Lindahl
Göran Dellgren
Anders Jeppsson
Julia Asp
Publicerad i Basic research in cardiology
Volym 107
Nummer/häfte 2
Sidor 255
ISSN 1435-1803
Publiceringsår 2012
Publicerad vid Institutionen för biomedicin, avdelningen för klinisk kemi och transfusionsmedicin
Institutionen för medicin
Institutionen för medicin, avdelningen för molekylär och klinisk medicin
Sidor 255
Språk en
Länkar dx.doi.org/10.1007/s00395-012-0255-...
Ämnesord Adult, Cell Differentiation, physiology, Cell Separation, Flow Cytometry, Gene Expression Profiling, Heart Atria, cytology, Humans, Myocardium, cytology, Real-Time Polymerase Chain Reaction, Side-Population Cells, cytology
Ämneskategorier Klinisk medicin

Sammanfattning

Cardiac "side population" (SP) cells have previously been found to differentiate into both endothelial cells and cardiomyocytes in mice and rats, but there are no data on SP cells in the human adult heart. Therefore, human cardiac atrial biopsies were dissociated, stained for SP cells and analyzed with FACS. Identified cell populations were analyzed for gene expression by quantitative real-time PCR and subjected to in vitro differentiation. Only biopsies from the left atrium contained a clearly distinguishable population of SP cells (0.22 ± 0.08%). The SP population was reduced by co-incubation with MDR1 inhibitor Verapamil, while the ABCG2 inhibitor FTC failed to decrease the number of SP cells. When the gene expression was analyzed, SP cells were found to express significantly more MDR1 than non-SP cells. For ABCG2, there was no detectable difference. SP cells also expressed more of the stem cell-associated markers C-KIT and OCT-4 than non-SP cells. On the other hand, no significant difference in the expression of endothelial and cardiac genes could be detected. SP cells were further subdivided based on CD45 expression. The CD45-SP population showed evidence of endothelial commitment at gene expression level. In conclusion, the results show that a SP population of cells is present also in the human adult heart.

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