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Skin Biopsies as Tools to Measure Fish Coloration and Colour Change

Artikel i övriga tidskrifter
Författare Helen Nilsson Sköld
P. Andreas Svensson
Publicerad i InTech
Sidor 1-18
Publiceringsår 2011
Publicerad vid Institutionen för marin ekologi
Sidor 1-18
Språk en
Länkar www.intechopen.com/articles/show/ti...
Ämnesord pigment, fish, color change, image analysis
Ämneskategorier Biologiska vetenskaper, Industriell bioteknik, Cell- och molekylärbiologi, Fysiologi, Morfologi

Sammanfattning

A biopsy is a link between the whole organism and its cells. Many recent advances in the field of fish coloration have been gained through experimentation on skin biopsies. In particular, this strategy has allowed the in-depth knowledge from pigment cell physiology to be applied to wider topics, such as behavioral ecology. Moreover, melanophores from fish and frogs have long served as models for a general understanding of intracellular transport and organelle positioning. For this purpose, fish skin biopsies have frequently been used for isolation of pigment cells but also for isolation of fibroblasts. In fish pigmentation research, skin biopsies have also been used to understand what causes changes in the skin's overall appearance, especially in the context of short or long-term modulation of tissue coloration. Examples of this are how many fishes rapidly change their colour during courtship or aggressive displays. Here, we review our methodology for the use of skin biopsies in research on fish coloration – from pigment cell physiology to animal behavior. We also highlight some of the most significant findings from this research, and present unsolved issues and suggestions for future directions. In this chapter, we will provide a description of the experimental procedures we have used to manipulate and analyze fish skin biopsies in the context of fish coloration - from chromatophores to animal behavior. Since our research has mainly focused on marine fishes, the protocols (e.g. the choice of buffers) were chosen to suit such animals. However, with minor modifications, these methods will likely work also on most fresh water species. Many of the experimental procedures are fast to assess and easy to perform, and we therefore encourage the experimentation on fish biopsies for use in education. The protocols described in this chapter are also suitable for such exercises.

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