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Interleukin-10 genotypes of the -1087 single nucleotide polymorphism influence Sp1 expression in periodontitis lesions

Artikel i vetenskaplig tidskrift
Författare Lena Larsson
Sara Thorbert-Mros
Lars Rymo
Tord Berglundh
Publicerad i Journal of Periodontology
Volym 82
Nummer/häfte 9
Sidor 1376-1382
ISSN 1943-3670
Publiceringsår 2011
Publicerad vid Institutionen för odontologi
Institutionen för biomedicin, avdelningen för klinisk kemi och transfusionsmedicin
Sidor 1376-1382
Språk en
Länkar dx.doi.org/10.1902/jop.2011.100623
Ämnesord cytokine, B cells, transcription factor, gene expression
Ämneskategorier Parodontologi

Sammanfattning

Background: Interleukin-10 (IL-10) is an important cytokine in immune regulation, and the -1087 IL-10 single nucleotide polymorphism (SNP) is associated with chronic periodontitis. The binding of the transcription factor Sp1 to the -1087 position in the IL-10 promoter up-regulates IL-10 gene expression, especially in patients with the GG genotype. A correlation between the -1087 GG genotype and high IL-10 and Sp1 gene expressions was found. Methods: Twenty-five individuals with severe, generalized chronic periodontitis were genotyped for the -1087 IL-10 gene polymorphism. SV40 promoter factor 1/specificity protein 1 (Sp1) and IL-10 mRNA were analyzed using real-time polymerase chain reaction. The amount of Sp1-positive cells and Sp1-positive B cells, as well as the amount of Sp1 protein, in periodontitis lesions were assessed using immunohistochemistry and in situ proximity ligation assay. Results: The mRNA expression of Sp1 and IL-10 in patients with the GG genotype was four times higher than that in patients with the AA genotype. The proportions of Sp1-positive cells overall and Sp1-positive B cells were larger in patients with the GG genotype than in patients with the AA genotype. Conclusions: The transcription factor Sp1 was present in large amounts in periodontitis lesions and the local expression of Sp1 was related to the -1087 IL-10 SNP.

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