Till sidans topp

Sidansvarig: Webbredaktion
Sidan uppdaterades: 2012-09-11 15:12

Tipsa en vän
Utskriftsversion

O-glycosylation of MUC1 m… - Göteborgs universitet Till startsida
Webbkarta
Till innehåll Läs mer om hur kakor används på gu.se

O-glycosylation of MUC1 mucin in prostate cancer and the effects of its expression on tumor growth in a prostate cancer xenograft model.

Artikel i vetenskaplig tidskrift
Författare Pushpa Premaratne
Karin Welén
Jan-Erik Damber
Gunnar C. Hansson
Malin Bäckström
Publicerad i Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine
Volym 32
Nummer/häfte 1
Sidor 203-13
ISSN 1423-0380
Publiceringsår 2011
Publicerad vid Institutionen för kliniska vetenskaper, Avdelningen för urologi
Institutionen för biomedicin, avdelningen för medicinsk kemi och cellbiologi
Sidor 203-13
Språk en
Länkar dx.doi.org/10.1007/s13277-010-0114-...
Ämnesord Animals, Blotting, Western, Cell Adhesion, Cell Movement, Cell Proliferation, Enzyme-Linked Immunosorbent Assay, Flow Cytometry, Glycosylation, Humans, Immunoenzyme Techniques, Male, Mice, Mice, Inbred BALB C, Mice, Nude, Mucin-1, metabolism, Prostatic Neoplasms, metabolism, pathology, Spectrometry, Mass, Electrospray Ionization, Tumor Cells, Cultured, Xenograft Model Antitumor Assays
Ämneskategorier Urologi och andrologi, Medicinsk bioteknologi (med inriktning mot cellbiologi (inklusive stamcellsbiologi), molekylärbiologi, mikrobiologi, biokemi eller biofarmaci)

Sammanfattning

MUC1 mucin is up-regulated and aberrantly glycosylated in many human epithelial carcinomas. Over-expression of MUC1 has also been implicated in prostate cancer, but neither the role of MUC1 in the cancer progression nor the mucin O-glycosylation has been fully elucidated. In this study, we characterized the O-glycans on MUC1 when over-expressed in the human prostate cancer cell line C4-2B(4). We found that the main O-glycan consisted of the neutral core 2 oligosaccharide Galβ3(Galβ3/4GlcNAcβ6)GalNAc-ol, with minor components being fucosylated and sialylated variants of the same core 2 oligosaccharide. Small amounts of the shorter core 1 O-glycans were also detected.We then used the MUC1 over-expressing cell lines to study the effects of MUC1 on prostate cancer cell behavior. The results demonstrate that over-expression of MUC1 did not affect the cells' proliferation, but led to a decreased adhesion to the extracellular matrix components fibronectin and collagen I in vitro. When inoculated in BALB/c nude mice, C4-2B(4) cells expressing MUC1 showed a tendency to form fewer tumors than wt cells and the tumors also grew more slowly, but there was a large variation between different tumors. These findings suggest that MUC1 may not have the same cancer-promoting effect in prostate cancer cells that is commonly seen in other epithelial cancers such as breast, colon, and pancreatic cancer.

Sidansvarig: Webbredaktion|Sidan uppdaterades: 2012-09-11
Dela:

På Göteborgs universitet använder vi kakor (cookies) för att webbplatsen ska fungera på ett bra sätt för dig. Genom att surfa vidare godkänner du att vi använder kakor.  Vad är kakor?