Till sidans topp

Sidansvarig: Webbredaktion
Sidan uppdaterades: 2012-09-11 15:12

Tipsa en vän
Utskriftsversion

Ghrelin decreases food in… - Göteborgs universitet Till startsida
Webbkarta
Till innehåll Läs mer om hur kakor används på gu.se

Ghrelin decreases food intake in juvenile rainbow trout (Oncorhynchus mykiss) through the central anorexigenic corticotropin-releasing factor system

Artikel i vetenskaplig tidskrift
Författare Elisabeth Jönsson
H Kaiya
Björn Thrandur Björnsson
Publicerad i General and Comparative Endocrinology
Volym 166
Nummer/häfte 166
Sidor 39-46
Publiceringsår 2010
Publicerad vid Zoologiska institutionen
Sidor 39-46
Språk en
Länkar dx.doi.org/10.1016/j.ygcen.2009.11....
Ämnesord Ghrelin; Teleosts; Salmonids; Appetite; Anorexia; Endocrine regulation; Body fat; CRF; CNS; Ghrelin receptor; GHS-R
Ämneskategorier Biologiska vetenskaper, Mikrobiologi, Zoofysiologi

Sammanfattning

Ghrelin stimulates pituitary growth hormone (GH) release, and has a key role in the regulation of food intake and adiposity in vertebrates. To investigate the central effect of native rainbow trout ghrelin (rtghrelin) on food intake in rainbow trout, as well as its possible mode of action, four groups of fish received a single injection into the third brain ventricle (i.c.v. injection): (1) control group (physiological saline) (2) ghrelin-treated group (2.0 ng rtghrelin g bwt−1), (3) group given the corticotropin-releasing hormone receptor antagonist α-helical CRF 9–41 (ahCRF) (4.0 ng g bwt−1) and (4) group receiving the same dose of both ghrelin and ahCRF. Food intake was assessed 1 h after treatment. In addition, the presence of the GHS-R (the ghrelin receptor) in the rainbow trout CNS was examined with Western blot. To investigate peripheral effects of ghrelin, rainbow trout received an intraperitoneal cholesterol-based implant with or without rtghrelin, and daily food intake was measured during 14 days. Weight and length were measured at the start and termination of the experiment and specific growth rates were calculated. Mesenteric fat stores, muscle and liver lipid content were analysed after the treatment period. Central ghrelin injections decreased food intake compared with controls, and treatment with ahCRF abolished the ghrelin-effect. Western blot analysis of the GHS-R revealed a single band at around 60 kDa in pituitary, hypothalamus, brain and stomach. Long-term peripheral ghrelin treatment decreased daily food intake compared with controls. This was reflected in a ghrelin-induced decrease in weight growth rate (p < 0.06). There was no effect of ghrelin on plasma GH levels or tissue fat stores. The conclusion from this study is that the GHS-R is indicated in the CNS in rainbow trout and that ghrelin may act there as an anorexigenic hormone, through a CRF-mediated pathway. Elevated peripheral ghrelin levels also seem to lead to decreased feed intake in the longer term.

Sidansvarig: Webbredaktion|Sidan uppdaterades: 2012-09-11
Dela:

På Göteborgs universitet använder vi kakor (cookies) för att webbplatsen ska fungera på ett bra sätt för dig. Genom att surfa vidare godkänner du att vi använder kakor.  Vad är kakor?