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Localization of O-glycans in MUC1 glycoproteins using electron-capture dissociation fragmentation mass spectrometry.

Artikel i vetenskaplig tidskrift
Författare Carina Sihlbom
Iris van Dijk Härd
Martin Lidell
Thomas Noll
Gunnar C. Hansson
Malin Bäckström
Publicerad i Glycobiology
Volym 19
Nummer/häfte 4
Sidor 375-81
ISSN 1460-2423
Publiceringsår 2009
Publicerad vid Institutionen för biomedicin, avdelningen för medicinsk kemi och cellbiologi
Sidor 375-81
Språk en
Länkar dx.doi.org/10.1093/glycob/cwn144
Ämnesord Acetylgalactosamine, chemistry, Animals, CHO Cells, Chromatography, Liquid, Cricetinae, Cricetulus, Glycosylation, Mass Spectrometry, Mucin-1, chemistry, genetics, Mutation, Peptides
Ämneskategorier Medicinsk bioteknologi (med inriktning mot cellbiologi (inklusive stamcellsbiologi), molekylärbiologi, mikrobiologi, biokemi eller biofarmaci)

Sammanfattning

MUC1 is a mucin glycoprotein containing multiple tandem repeats of 20 amino acids, with five serines and threonines that can be O-glycosylated. Here, we investigated the O-glycosylation site occupancy in MUC1 glycoproteins produced in two mutant CHO cell lines, Lec3.2.8.1 and ldlD. We found that the average site occupancy was higher in MUC1 from Lec3.2.8.1 than from ldlD and that the occupancy increased with the number of tandem repeats in the protein and also depended on the culture conditions used for production. Moreover, we describe the successful use of electron-capture dissociation (ECD) fragmentation, coupled to online liquid chromatography mass spectrometry, to determine the glycosylation of individual sites in recombinant MUC1 proteins with 16 tandem repeats. We analyzed MUC1 tandem repeat peptides with 1-5 GalNAc residues by ECD fragmentation and found that the first site to be glycosylated was either Ser-5 or Thr-6, with the addition of a second GalNAc at Thr-14. For peptides with three GalNAc residues, several different variants of glycopeptides were found, indicating a heterogeneous order of glycosylation at this stage. In contrast, only one variant was found for peptides with four GalNAc residues, where Thr-19 in the PDTR motif was left unglycosylated, indicating that this site is glycosylated last. The results gave novel insight into the order of GalNAc substitution in MUC1 in vivo.

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