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Calpain processing of brain microtubules from the Atlantic cod, Gadus morhua.

Artikel i vetenskaplig tidskrift
Författare Martin Billger
E Nilsson
Jan-Olof Karlsson
Margareta Wallin
Publicerad i Molecular and cellular biochemistry
Volym 121
Nummer/häfte 1
Sidor 85-92
ISSN 0300-8177
Publiceringsår 1993
Publicerad vid Zoologiska institutionen
Institutionen för anatomi och cellbiologi
Sidor 85-92
Språk en
Länkar www.ncbi.nlm.nih.gov/entrez/query.f...
Ämnesord Animals, Brain Chemistry, Calpain, analysis, pharmacology, Electrophoresis, Polyacrylamide Gel, Fishes, Microscopy, Electron, Microtubule-Associated Proteins, metabolism, Microtubules, drug effects, metabolism, ultrastructure, Muscles, chemistry, Rabbits
Ämneskategorier Cell- och molekylärbiologi

Sammanfattning

Microtubules isolated from Atlantic cod (Gadus morhua) brains retained assembly competence and ultraculture, although treatment with rabbit calpain resulted in loss of MAPs. In addition, spirals and aberrant structures formed when calpain I was activated post assembly. No such effect was seen with calpain II. Soluble fractions from cod brain were found to contain proteolytic activity that could be blocked by exogenously added calpastatin. Calpain was also isolated from cod muscle tissue with 10 times less yield, compared to rabbit lung. On the basis of Ca(2+)-requirements for activation in the mM range, electrophoretic mobility, antigenicity and hydrophobicity, we conclude that the proteolytic activity was attributable to calpain II. There was no difference in effects of rabbit and cod calpain II on cod microtubule proteins, indicating that calpain is a conserved protein. Our results suggest that calpains might be involved in the Ca(2+)-dependent irreversible regulation of cod brain microtubules.

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