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Effect of circadian rhythm, age, training and acute lameness on serum concentrations of cartilage oligomeric matrix protein (COMP) neo-epitope in horses

Artikel i vetenskaplig tidskrift
Författare S. Ekman
Anders Lindahl
Ulla Ruetschi
A. Jansson
K. Bjorkman
K. Abrahamsson-Aurell
S. Bjornsdottir
M. Lofgren
Lillemor Mattsson Hultén
Eva Skiöldebrand
Publicerad i Equine Veterinary Journal
Volym 51
Nummer/häfte 5
Sidor 674-680
ISSN 0425-1644
Publiceringsår 2019
Publicerad vid Institutionen för biomedicin, avdelningen för klinisk kemi och transfusionsmedicin
Sidor 674-680
Språk en
Länkar dx.doi.org/10.1111/evj.13082
Ämnesord horse, COMP neo-epitope, biomarker, lameness, serum, training, circadian, synovial-fluid, joint disease, biomarker, inflammation, collagen, urine, bone, Veterinary Sciences
Ämneskategorier Invärtesmedicin

Sammanfattning

Background Molecular serum markers that can identify early reversible osteoarthritis (OA) in horses are lacking. Objectives We studied serum concentrations of a novel cartilage oligomeric matrix protein (COMP) neo-epitope in horses subjected to short-term exercise and with acute lameness. The effects of circadian rhythm and age were also evaluated. Study design Longitudinal studies in healthy horses and cross-sectional comparison of lame and non-lame horses. Methods Sera were collected from five horses before and after short-term interval exercise and during full-day box rest. Sera from 32 acutely lame horses were used to evaluate age-related effects. Independent samples from control horses (n = 41) and horses with acute lameness (n = 71) were included. COMP neo-epitope concentrations were analysed using custom-developed inhibition ELISAs validated for equine serum. The presence of COMP neo-epitope was delineated in healthy and osteoarthritic articular cartilage with immunohistochemistry. Results COMP neo-epitope concentrations decreased after speed training but returned to baseline levels post-exercise. No correlations between age and serum COMP neo-epitope concentrations were found (r = 0.0013). The mean (+/- s.d.) serum concentration of COMP neo-epitope in independent samples from non-lame horses was 0.84 +/- 0.38 mu g/mL, and for lame horses was 5.24 +/- 1.83 mu g/mL (P<0.001). Antibodies against COMP neo-epitope did not stain normal articular cartilage, but intracytoplasmic staining was found in superficial chondrocytes of mild OA cartilage and in the extracellular matrix of moderately osteoarthritic cartilage. Main limitations ELISA was based on polyclonal antisera rather than a monoclonal antibody. There is a sex and breed bias within the groups of horses, also it could have been of value to include horses with septic arthritis and tendonitis and investigated joint differences. Conclusions This COMP neo-epitope can be measured in sera, and results indicate that it could be a biomarker for pathologic fragmentation of cartilage in connection with acute joint lameness.

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Denna text är utskriven från följande webbsida:
http://www.gu.se/forskning/publikation/?publicationId=282802
Utskriftsdatum: 2019-12-12