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Recovery of mutans streptococci on MSB, SB-20 and SB-20M agar media

Artikel i vetenskaplig tidskrift
Författare Marta Estela Saravia
Paulo Nelson-Filho
Raquel Assed Bezerra Silva
Andiara De Rossi
Gisele Faria
Léa Assed Bezerra Silva
Claes-Göran Emilson
Publicerad i Archives of Oral Biology
Volym 58
Sidor 311-316
ISSN 00039969
Publiceringsår 2013
Publicerad vid Institutionen för odontologi
Sidor 311-316
Språk en
Länkar doi.org/10.1016/j.archoralbio.2012....
Ämnesord Selective culture media, Streptococcus mutans, Streptococcus sobrinus
Ämneskategorier Odontologi

Sammanfattning

Objective: The recovery of mutans streptococci in saliva and dental biofilm samples depends, in part, on the culture medium used. In this study, we compared (i) the culture media Sucrose-Bacitracin agar (SB-20), Modified SB-20 (SB-20M) and Mitis Salivarius Bacitracin agar (MSB) in the count of colony forming units (cfu) of mutans streptococci and (ii) in the morphological and biochemical differentiation between Streptococcus mutans and Streptococcus sobrinus. Design: Samples of non-stimulated saliva from 20 children were plated on SB-20, SB-20M and MSB, and incubated in microaerophilia at 37 °C for 72 h. Identification of microorganisms was based on analysis of colony morphology under stereomicroscopy. The biochemical identification of colonies was done by biochemical tests using sugar fermentation, resistance to bacitracin and hydrogen peroxide production. Results: There was no significant difference (p > 0.05) in the number of cfu of mutans streptococci recovered on SB-20 and SB-20M agar. Comparing the media, SB-20 and SB-20M yielded a larger number of mutans streptococci colonies (p < 0.05) and were more effective than MSB in the identification of S. sobrinus (p < 0.05), but not of S. mutans (p > 0.05). Conclusion: There was no significant difference between SB-20 and SB-20M culture media in the count of mutans streptococci, demonstrating that the replacement of sucrose by coarse granular cane sugar did not alter the efficacy of the medium. Compared with MSB, SB-20 and SB-20M allowed counting a larger number of mutans streptococci colonies and a more effective morphological identification of S. sobrinus. © 2012 Elsevier Ltd.

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Utskriftsdatum: 2019-09-15