Till sidans topp

Sidansvarig: Webbredaktion
Sidan uppdaterades: 2012-09-11 15:12

Tipsa en vän
Utskriftsversion

Nuclear factor 1-C2 contr… - Göteborgs universitet Till startsida
Webbkarta
Till innehåll Läs mer om hur kakor används på gu.se

Nuclear factor 1-C2 contributes to the tissue-specific activation of a milk protein gene in the differentiating mammary gland.

Artikel i vetenskaplig tidskrift
Författare Marie Kannius-Janson
Eva M. Johansson
Gunnar Bjursell
Jeanette Nilsson
Publicerad i The Journal of biological chemistry
Volym 277
Nummer/häfte 20
Sidor 17589-96
ISSN 0021-9258
Publiceringsår 2002
Publicerad vid Institutionen för cell- och molekylärbiologi
Sidor 17589-96
Språk en
Länkar dx.doi.org/10.1074/jbc.M105979200
Ämnesord Alternative Splicing, Animals, Blotting, Western, Breast, growth & development, CCAAT-Enhancer-Binding Proteins, physiology, Carboxylesterase, Carboxylic Ester Hydrolases, genetics, Cell Differentiation, DNA-Binding Proteins, Female, Gene Expression Regulation, Mice, Mice, Inbred C57BL, Mice, Inbred CBA, Milk Proteins, genetics, NFI Transcription Factors, Nuclear Proteins, Rats, Transcription Factors, Transfection, Tumor Cells, Cultured, Y-Box-Binding Protein 1
Ämneskategorier Biologiska vetenskaper

Sammanfattning

Members of the nuclear factor 1 (NF1) transcription factor family have been postulated to be involved in the regulation of milk genes. In this work we have been able to identify the splice variant NF1-C2 as an important member of a tissue-specific activating complex that regulates the milk gene encoding carboxyl ester lipase (CEL). Mutation of the NF1-binding site in the CEL gene promoter results in a drastic reduction of the gene expression to about 15% in mammary epithelial cells. Furthermore, we demonstrate that the NF1-C2 protein interacts with a higher affinity to the NF1-binding site in the CEL gene promoter than other NF1 family members do and that NF1-C2 in the mouse mammary gland is a phosphorylated protein. During development of the mouse mammary gland, binding of NF1-C2 to the CEL gene promoter is induced at midpregnancy, in correlation with the induction of CEL gene expression. The fact that the NF1-C2 involving complex remains throughout the lactation period and decreases during the weaning period, when the CEL gene is down-regulated, supports its importance in the regulation of CEL gene expression. To our knowledge, this is the first report identifying a specific, endogenously expressed NF1 isoform to be involved in the tissue-specific activation of a gene.

Sidansvarig: Webbredaktion|Sidan uppdaterades: 2012-09-11
Dela:

På Göteborgs universitet använder vi kakor (cookies) för att webbplatsen ska fungera på ett bra sätt för dig. Genom att surfa vidare godkänner du att vi använder kakor.  Vad är kakor?

Denna text är utskriven från följande webbsida:
http://www.gu.se/forskning/publikation/?publicationId=240429
Utskriftsdatum: 2020-02-18