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Interaction between Na+/K+-pump and Na+/Ca2+-exchanger modulates intercellular communication.

Artikel i vetenskaplig tidskrift
Författare Vladimir V Matchkov
Helena Gustafsson
Awahan Rahman
Donna M Briggs Boedtkjer
Sarah Gorintin
Anne Kirstine Hansen
Elena V Bouzinova
Helle A Praetorius
Christian Aalkjaer
Holger Nilsson
Publicerad i Circulation research
Volym 100
Nummer/häfte 7
Sidor 1026-35
ISSN 1524-4571
Publiceringsår 2007
Publicerad vid Institutionen för neurovetenskap och fysiologi, sektionen för fysiologi
Sidor 1026-35
Språk en
Länkar dx.doi.org/10.1161/01.RES.000026265...
Ämnesord Aniline Compounds, pharmacology, Animals, Aorta, cytology, metabolism, Calcium, metabolism, Cell Communication, physiology, Cell Membrane, physiology, Cells, Cultured, Drug Interactions, Electric Capacitance, Enzyme Inhibitors, pharmacology, Intracellular Membranes, metabolism, Male, Mesenteric Arteries, cytology, metabolism, Muscle, Smooth, Vascular, physiology, Myocytes, Smooth Muscle, drug effects, physiology, Osmolar Concentration, Ouabain, pharmacology, Phenyl Ethers, pharmacology, Potassium Channel Blockers, pharmacology, Rats, Rats, Wistar, Sodium-Calcium Exchanger, antagonists & inhibitors, physiology, Sodium-Potassium-Exchanging ATPase, antagonists & inhibitors, physiology, Tissue Distribution, Vasoconstriction, drug effects
Ämneskategorier Medicin och Hälsovetenskap

Sammanfattning

Ouabain, a specific inhibitor of the Na(+)/K(+)-pump, has previously been shown to interfere with intercellular communication. Here we test the hypothesis that the communication between vascular smooth muscle cells is regulated through an interaction between the Na(+)/K(+)-pump and the Na(+)/Ca(2+)-exchanger leading to an increase in the intracellular calcium concentration ([Ca(2+)](i)) in discrete areas near the plasma membrane. [Ca(2+)](i) in smooth muscle cells was imaged in cultured rat aortic smooth muscle cell pairs (A7r5) and in rat mesenteric small artery segments simultaneously with force. In A7r5 coupling between cells was estimated by measuring membrane capacitance. Smooth muscle cells were uncoupled when the Na(+)/K(+)-pump was inhibited either by a low concentration of ouabain, which also caused a localized increase of [Ca(2+)](i) near the membrane, or by ATP depletion. Reduction of Na(+)/K(+)-pump activity by removal of extracellular potassium ([K(+)](o)) also uncoupled cells, but only after inhibition of K(ATP) channels. Inhibition of the Na(+)/Ca(2+)-exchange activity by SEA0400 or by a reduction of the equilibrium potential (making it more negative) also uncoupled the cells. Depletion of intracellular Na(+) and clamping of [Ca(2+)](i) at low concentrations prevented the uncoupling. The experiments suggest that the Na(+)/K(+)-pump may affect gap junction conductivity via localized changes in [Ca(2+)](i) through modulation of Na(+)/Ca(2+)-exchanger activity.

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