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Imaging TOF-SIMS of rat kidney prepared by high-pressure freezing.

Artikel i vetenskaplig tidskrift
Författare Håkan Nygren
Katrin Börner
Per Malmberg
Elke Tallarek
Birgit Hagenhoff
Publicerad i Microscopy research and technique
Volym 68
Nummer/häfte 6
Sidor 329-34
ISSN 1059-910X
Publiceringsår 2005
Publicerad vid Institutionen för anatomi och cellbiologi
Sidor 329-34
Språk en
Länkar dx.doi.org/10.1002/jemt.20258
Ämnesord Animals, Atmospheric Pressure, Cytological Techniques, instrumentation, methods, Diagnostic Imaging, instrumentation, methods, Freezing, Image Processing, Computer-Assisted, Kidney, cytology, metabolism, Rats, Spectrometry, Mass, Secondary Ion, methods
Ämneskategorier Medicin och Hälsovetenskap

Sammanfattning

Phosphocholine, potassium ions, and sodium ions were localized in rat kidney with imaging TOF-SIMS. Tissue preparation was performed with high-pressure freezing, freeze-fracturing and freeze-drying. The distribution of sodium ions was visualized by imaging the signal at m/z 23 of positively charged secondary ions, and the distribution of potassium ions was visualized by imaging the signal at m/z 39. Potassium was found localized within cells of the proximal tubulus epithelium and within cells of the glomeruli. High signals of sodium ions were seen in the interstitial tissue and also in epithelial cells of the collecting ducts and in glomeruli. The overlay image showed that the distribution of sodium ions and potassium ions were largely complementary with color mixing in glomeruli and in the interstitium surrounding proximal tubules. The ion distribution was further analyzed by correlation analysis. Phosphocholine-containing phospholipids were visualized by imaging the phosphocholine head group at m/z 184 of positively charged ions. The m/z 184 signal shows a ubiquitous distribution with a high intensity of phosphocholine in epithelial cells. Overlay image of m/z 184, m/z 39, and m/z 23 and multivariate analysis showed that the localization of high levels of phosphocholine colocalizes with high levels of potassium ions, as expected for an ion with intracellular localization.

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Utskriftsdatum: 2020-08-11