Till sidans topp

Sidansvarig: Webbredaktion
Sidan uppdaterades: 2012-09-11 15:12

Tipsa en vän
Utskriftsversion

Nonlinear microscopy of l… - Göteborgs universitet Till startsida
Webbkarta
Till innehåll Läs mer om hur kakor används på gu.se

Kontaktformulär








 


OBS! Vill du ha svar, ange e-post eller telefonnummer!




Nonlinear microscopy of lipid storage and fibrosis in muscle and liver tissues of mice fed high-fat diets

Artikel i vetenskaplig tidskrift
Författare Christian Brackmann
Britt G. Gabrielsson
Fredrik Svedberg
Agneta Holmäng
Ann-Sofie Sandberg
Annika Enejder
Publicerad i Journal of Biomedical Optics
Volym 15
Nummer/häfte 6
Sidor 066008-1 - 066008-10
ISSN 1083-3668
Publiceringsår 2010
Publicerad vid Institutionen för neurovetenskap och fysiologi, sektionen för fysiologi
Sidor 066008-1 - 066008-10
Språk en
Länkar dx.doi.org/10.1117/1.3505024
https://gup.ub.gu.se/file/148624
Ämnesord cellular biophysics, coherent antiStokes Raman scattering, diseases, harmonic generation, lipid bilayers, liver, muscle, optical microscopy
Ämneskategorier Medicinsk teknik, Molekylär medicin (genetik och patologi), Endokrinologi

Sammanfattning

Hallmarks of high-fat Western diet intake, such as excessive lipid accumulation in skeletal muscle and liver as well as liver fibrosis, are investigated in tissues from mice using nonlinear microscopy, second harmonic generation (SHG), and coherent anti-Stokes Raman scattering (CARS), supported by conventional analysis methods. Two aspects are presented; intake of standard chow versus Western diet, and a comparison between two high-fat Western diets of different polyunsaturated lipid content. CARS microscopy images of intramyocellular lipid droplets in muscle tissue show an increased amount for Western diet compared to standard diet samples. Even stronger diet impact is found for liver samples, where combined CARS and SHG microscopy visualize clear differences in lipid content and collagen fiber development, the latter indicating nonalcoholic fatty liver disease (NAFLD) and steatohepatitis induced at a relatively early stage for Western diet. Characteristic for NAFLD, the fibrous tissue-containing lipids accumulate in larger structures. This is also observed in CARS images of liver samples from two Western-type diets of different polyunsaturated lipid contents. In summary, nonlinear microscopy has strong potential (further promoted by technical advances toward clinical use) for detection and characterization of steatohepatitis already in its early stages.

Sidansvarig: Webbredaktion|Sidan uppdaterades: 2012-09-11
Dela:

På Göteborgs universitet använder vi kakor (cookies) för att webbplatsen ska fungera på ett bra sätt för dig. Genom att surfa vidare godkänner du att vi använder kakor.  Vad är kakor?