Currently, most eco-relevant toxicology data on Nanomaterials (NMs) are limited to species used in regulatory testing using standardised tests methods skewed towards high concentrations and short term (24-48h) exposures. These tests however, may not currently be ideal for evaluating toxicity of NMs. Work package 3 of the MISTRA ENVIRONMENTAL NANOSAFETY PROJECT- Phase II is tasked at adapting available and developing new acute and long term testing protocols to assess effects associated with NM exposure considering conditions of weathering in comparison to pristine NMs.
A range of metal and plastic nanomaterials will be evaluated for changes in toxicity patterns as a result of weathering cause by intentional addition of Natural organic matter (NOM). Focus will be placed on evaluating toxicity via In vitro assays using Rainbow trout cell models (2D vs.3D), zebrafish embryo and larvae tests and eventually in vivo studies to determine the effects on food chains. Endpoints to be assessed may include lethal (Cell death, mortality) and sub-lethal (behavioural changes, gene/protein expression analysis, ROS generation, hormone levels etc.).