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Activation of a cGMP-sensitive calcium-dependent chloride channel may cause transition from calcium waves to whole cell oscillations in smooth muscle cells.

Journal article
Authors Jens Christian Brings Jacobsen
Christian Aalkjaer
Holger Nilsson
Vladimir V Matchkov
Jacob Freiberg
Niels-Henrik Holstein-Rathlou
Published in American journal of physiology. Heart and circulatory physiology
Volume 293
Issue 1
Pages H215-28
ISSN 0363-6135
Publication year 2007
Published at Institute of Neuroscience and Physiology, Department of Physiology
Pages H215-28
Language en
Links dx.doi.org/10.1152/ajpheart.00726.2...
Keywords Adaptation, Physiological, physiology, Animals, Biological Clocks, physiology, Calcium, metabolism, Calcium Signaling, physiology, Cells, Cultured, Chloride Channels, physiology, Computer Simulation, Cyclic GMP, metabolism, Humans, Ion Channel Gating, physiology, Models, Cardiovascular, Muscle, Smooth, Vascular, physiology, Myocytes, Smooth Muscle, physiology
Subject categories Medical and Health Sciences

Abstract

In vitro, alpha-adrenoreceptor stimulation of rat mesenteric small arteries often leads to a rhythmic change in wall tension, i.e., vasomotion. Within the individual smooth muscle cells of the vascular wall, vasomotion is often preceded by a period of asynchronous calcium waves. Abruptly, these low-frequency waves may transform into high-frequency whole cell calcium oscillations. Simultaneously, multiple cells synchronize, leading to rhythmic generation of tension. We present a mathematical model of vascular smooth muscle cells that aims at characterizing this sudden transition. Simulations show calcium waves sweeping through the cytoplasm when the sarcoplasmic reticulum (SR) is stimulated to release calcium. A rise in cGMP leads to the experimentally observed transition from waves to whole cell calcium oscillations. At the same time, membrane potential starts to oscillate and the frequency approximately doubles. In this transition, the simulated results point to a key role for a recently discovered cGMP-sensitive calcium-dependent chloride channel. This channel depolarizes the membrane in response to calcium released from the SR. In turn, depolarization causes a uniform opening of L-type calcium channels on the cell surface, stimulating a synchronized release of SR calcium and inducing the shift from waves to whole cell oscillations. The effect of the channel is therefore to couple the processes of the SR with those of the membrane. We hypothesize that the shift in oscillatory mode and the associated onset of oscillations in membrane potential within the individual cell may underlie sudden intercellular synchronization and the appearance of vasomotion.

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