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Isolation and characterization of a novel simazine-degrading bacterium from agricultural soil of central Chile, Pseudomonas sp MHP41

Journal article
Authors Marcela Hernandez
Patricio Villalobos
Veronica Morgante
Myriam Gonzalez
Caroline Reiff
Edward R.B. Moore
Michael Seeger
Published in Fems Microbiology Letters
Volume 286
Issue 2
Pages 184-190
ISSN 0378-1097
Publication year 2008
Published at Institute of Biomedicine, Department of Infectious Medicine
Pages 184-190
Language en
Keywords burkholderia-xenovorans lb400, atrazine catabolism genes, sp strain adp, nucleotide-sequence, stress-proteins, degradation, organization, pathway, mineralization, versatility
Subject categories Microbiology


s-Triazine herbicides are used extensively in South America in agriculture and forestry. In this study, a bacterium designated as strain MHP41, capable of degrading simazine and atrazine, was isolated from agricultural soil in the Quillota valley, central Chile. Strain MHP41 is able to grow in minimal medium, using simazine as the sole nitrogen source. In this medium, the bacterium exhibited a growth rate of μ=0.10 h−1, yielding a high biomass of 4.2 × 108 CFU mL−1. Resting cells of strain MHP41 degrade more than 80% of simazine within 60 min. The atzA, atzB, atzC, atzD, atzE and atzF genes encoding the enzymes of the simazine upper and lower pathways were detected in strain MHP41. The motile Gram-negative bacterium was identified as a Pseudomonas sp., based on the Biolog microplate system and comparative sequence analyses of the 16S rRNA gene. Amplified ribosomal DNA restriction analysis allowed the differentiation of strain MHP41 from Pseudomonas sp. ADP. The comparative 16S rRNA gene sequence analyses suggested that strain MHP41 is closely related to Pseudomonas nitroreducens and Pseudomonas multiresinovorans. This is the first s-triazine-degrading bacterium isolated in South America. Strain MHP41 is a potential biocatalyst for the remediation of s-triazine-contaminated environments.

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