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Neuroendocrine secretory protein 55 (NESP55) in the spinal cord of rat: an immunocytochemical study.

Journal article
Authors Yongling Li
Reiner Fischer-Colbrie
Annica Dahlström
Published in The Journal of comparative neurology
Volume 506
Issue 4
Pages 733-44
ISSN 0021-9967
Publication year 2008
Published at Institute of Biomedicine, Department of Medical Biochemistry and Cell Biology
Pages 733-44
Language en
Links dx.doi.org/10.1002/cne.21562
Keywords Acetylcholine, metabolism, Animals, Autonomic Nervous System, cytology, metabolism, Axons, metabolism, ultrastructure, Cytoplasm, metabolism, ultrastructure, Female, GTP-Binding Protein alpha Subunits, Gs, metabolism, Immunohistochemistry, Interneurons, cytology, metabolism, Male, Membrane Glycoproteins, metabolism, Motor Neurons, cytology, metabolism, Neurons, cytology, metabolism, Neurosecretion, physiology, Rats, Rats, Sprague-Dawley, Secretory Vesicles, metabolism, ultrastructure, Spinal Cord, cytology, metabolism
Subject categories Histology

Abstract

The immunohistochemical expression of a novel chromogranin-like protein, neuroendocrine secretory protein 55 (NESP55), in the rat spinal cord was investigated. NESP55-immunoreactive cells were detected in the ventral horn, intermediate laminae, and deep dorsal horn, comprising motoneurons, autonomic neurons, and interneurons throughout all spinal segments. Within laminae I-II of the dorsal horn, one or two NESP55-positive cells were often seen. Nerve fibers also contained NESP55 immunoreactivity (IR) and were particularly prominent in the ventral horn. No nerve terminals/varicosities appeared to contain NESP55 in any spinal lamina. Double-staining experiments revealed that a high proportion of the NESP55-positive neurons were cholinergic. Moreover, NESP55-IR in the motoneurons was evenly distributed in the whole cytoplasm with a finely granular appearance. In contrast, the fluorescent material in the preganglionic neurons was concentrated in the perinuclear region and largely overlapped with the trans-Golgi network marker TGN38. Our data provide detailed morphological information on the distribution of NESP55-IR in the rat spinal cord. Also, the differential intracellular expression of NESP55-IR in the spinal motoneurons and autonomic neurons suggests that NESP55 may be processed into different secretory granules and may be involved in both constitutive and regulated pathways in these neurons.

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