To the top

Page Manager: Webmaster
Last update: 9/11/2012 3:13 PM

Tell a friend about this page
Print version

Quantification and locali… - University of Gothenburg, Sweden Till startsida
To content Read more about how we use cookies on

Quantification and localization of the IGF/insulin system expression in retinal blood vessels and neurons during oxygen-induced retinopathy in mice

Journal article
Authors Chatarina Löfqvist
K. L. Willett
Oskar Aspegren
A. C. Smith
C. M. Aderman
K. M. Connor
J. Chen
Ann Hellström
L. E. Smith
Published in Invest Ophthalmol Vis Sci
Volume 50
Issue 4
Pages 1831-1837
ISSN 1552-5783
Publication year 2009
Published at Institute of Neuroscience and Physiology, Department of Clinical Neuroscience and Rehabilitation
Pages 1831-1837
Language en
Keywords Animals, DNA Primers/chemistry, Disease Models, Animal, Gene Expression Regulation, Humans, Infant, Newborn, Insulin-Like Growth Factor Binding Proteins/*genetics, Insulin-Like Growth Factor I/*genetics, Insulin-Like Growth Factor II/genetics, Mice, Mice, Inbred C57BL, Oxygen/*toxicity, Photoreceptor Cells, Vertebrate/metabolism, RNA, Messenger/metabolism, Receptor, IGF Type 1/*genetics, Receptor, IGF Type 2/genetics, Receptor, Insulin/*genetics, Retinal Neurons/*metabolism, Retinal Vessels/*metabolism, Retinopathy of Prematurity/chemically induced/metabolism, Reverse Transcriptase Polymerase Chain Reaction
Subject categories Medical and Health Sciences


PURPOSE: Retinopathy is a result of pathologic angiogenesis influenced by insulinlike growth factor (IGF)-1. The authors examined the local expression of the IGF/insulin family. METHODS: In retinas with and without oxygen-induced retinopathy, the authors assessed with real-time RT-PCR mRNA expression of the IGF-1 receptor (IGF-1R), insulin receptor (IR), IGF-1, IGF-2, insulin (Ins2), and IGF-binding protein 1 (IGFBP1) to IGFBP6 in total retina from postnatal day (P) 7 to P33 to examine changes over time with the induction of retinopathy and at P17 on laser-captured retinal components to quantitatively localize mRNA expression in the ganglion cell layer, the outer nuclear layer, the inner nuclear layer, normal blood vessels, and neovascular tufts. RESULTS: IGF-1R and IR are expressed predominantly in photoreceptors and in vessels, with scant expression in the rest of the neural retina. IGF-1R expression is more than 100-fold greater than IR. The major local growth factor (expressed in photoreceptors and in blood vessels) is IGF-2 (approximately 1000-fold greater than IGF-1). IGF-1 (approximately 600 copies/10(6) cyclophilin) is expressed throughout the retina. IGFBP2, IGFBP4, and IGFBP5 expression is unchanged with increasing retinal development and with the induction of retinopathy. In contrast, IGFBP3 expression increased more than 5-fold with hypoxia, found in neovascular tufts. CONCLUSIONS: IGF-1R, IR, and the ligand IGF-2 are expressed almost exclusively in photoreceptors and blood vessels. IGFBP3 and IGFBP5 expression increases in neovascular tufts compared with normal vessels. IGF-1 is expressed throughout the retina at much lower levels. These results suggest cross-talk between vessels and photoreceptors in the development of retinopathy and retinal vasculature.

Page Manager: Webmaster|Last update: 9/11/2012

The University of Gothenburg uses cookies to provide you with the best possible user experience. By continuing on this website, you approve of our use of cookies.  What are cookies?