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Expression of functional growth hormone receptors in cultured human osteoblast-like cells.

Journal article
Authors Anders Nilsson
Diana Swolin-Eide
Sven Enerbäck
Claes Ohlsson
Published in The Journal of clinical endocrinology and metabolism
Volume 80
Issue 12
Pages 3483-8
ISSN 0021-972X
Publication year 1995
Published at Institute of Medical Biochemistry
Institute of Surgical Sciences, Department of Orthopaedics
Institute of Internal Medicine, Dept of Medicine
Institute for the Health of Women and Children, Dept of Paediatrics
Pages 3483-8
Language en
Links www.ncbi.nlm.nih.gov/entrez/query.f...
Keywords Base Sequence, Cells, Cultured, Growth Hormone, metabolism, Humans, Molecular Probes, genetics, Molecular Sequence Data, Osteoblasts, metabolism, RNA, Messenger, metabolism, Receptors, Somatotropin, genetics, metabolism, Thymidine, metabolism
Subject categories Medical and Health Sciences

Abstract

Recent clinical studies indicate that GH is important for bone remodeling. Patients with GH deficiency exhibit decreased bone density, and GH substitution increases bone density in these patients. The aim of the present study was to investigate the presence of GH receptors and the effects of GH on cultured human osteoblast-like cells. Primary cultures of human osteoblast-like cells were established from trabecular bone. Northern blot analysis, using a probe recognizing exon 10 of the human GH receptor, revealed a 4.7-kilobase transcript corresponding to the human GH receptor. Cultured osteoblast-like cells expressed, as determined by RNase protection assay, approximately one fourth of the GH receptor messenger RNA levels found in liver. Binding studies using 125I-labeled GH revealed a single class of receptors with approximately 2000 binding sites per cell and an association constant (Ka) of 2.6 x 10(9) M-1. GH stimulation of the cultured cells resulted in increased [3H]thymidine incorporation, suggesting that the GH receptors are functional. In summary, the present study shows that cultured human osteoblast-like cells express functional GH receptors.

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