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Calibration of a hand-held haemoglobin analyser for use on fish blood

Journal article
Authors T.D. Clark
E.J. Eliason
Erik Sandblom
S.G. Hinch
A. P. Farrell
Published in Journal of Fish Biology
Volume 73
Issue 10
Pages 2587-2595
ISSN 0022-1112
Publication year 2008
Published at Department of Zoology
Pages 2587-2595
Language en
Keywords Drabkin's method, haemoglobin concentration, haemoglobinometer, HemoCue Hb 201+, spectrophotometer
Subject categories Animal physiology


The HemoCue haemoglobin analyser consistently overestimated haemoglobin concentration ([Hb]) in the blood of all fish species (sockeye salmon Oncorhynchus nerka, Chinook salmon Oncorhynchus tshawytscha, Pacific bluefin tuna Thunnus orientalis and chub mackerel Scomber japonicus) by 22–50% (9·9–36·0 g l−1) over a [Hb] range of 20–160 g l−1. The systematic nature of the overestimation, however, allowed the formulation of an accurate calibration equation that can be used to correct values of [Hb] measured by the HemoCue in field studies.

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