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Five mucosal transcripts of interest in ulcerative colitis identified by quantitative real-time PCR: a prospective study.

Journal article
Authors Anders Eriksson
Carl-Fredrik Flach
Anders Lindgren
Eva Kvifors
Stefan Lange
Published in BMC gastroenterology
Volume 8
Pages 34
ISSN 1471-230X
Publication year 2008
Published at Institute of Biomedicine
Institute of Biomedicine, Department of Microbiology and Immunology
Institute of Medicine, Department of Emergeny and Cardiovascular Medicine
Institute of Biomedicine, Department of Infectious Medicine
Pages 34
Language en
Links dx.doi.org/10.1186/1471-230X-8-34
Keywords Adult, Aged, Aged, 80 and over, Amino Acid Transport Systems, Neutral, genetics, metabolism, Biopsy, Carrier Proteins, genetics, metabolism, Case-Control Studies, Cecum, metabolism, pathology, Colitis, Ulcerative, metabolism, pathology, Elafin, genetics, metabolism, Female, Fructose-Bisphosphate Aldolase, genetics, metabolism, Hepatocyte Growth Factor, genetics, metabolism, Humans, Male, Middle Aged, Mucous Membrane, metabolism, pathology, Polymerase Chain Reaction, Prospective Studies, Proto-Oncogene Proteins, genetics, metabolism, Rectum, metabolism, pathology, Transcription, Genetic
Subject categories Molecular biology, Molecular biology

Abstract

BACKGROUND: The cause and pathophysiology of ulcerative colitis are both mainly unknown. We have previously used whole-genome microarray technique on biopsies obtained from patients with ulcerative colitis to identify 5 changed mucosal transcripts. The aim of this study was to compare mucosal expressions of these five transcripts in ulcerative colitis patients vs. controls, along with the transcript expression in relation to the clinical ulcerative colitis status. METHODS: Colonic mucosal specimens from rectum and caecum were taken at ambulatory colonoscopy from ulcerative colitis patients (n = 49) with defined inflammatory activity and disease extension, and from controls (n = 67) without inflammatory bowel disease. The five mucosal transcripts aldolase B, elafin, MST-1, simNIPhom and SLC6A14 were analyzed using quantitative real-time PCR. RESULTS: Significant transcript differences in the rectal mucosa for all five transcripts were demonstrated in ulcerative colitis patients compared to controls. The grade of transcript expression was related to the clinical disease activity. CONCLUSION: The five gene transcripts were changed in patients with ulcerative colitis, and were related to the disease activity. The known biological function of some of the transcripts may contribute to the inflammatory features and indicate a possible role of microbes in ulcerative colitis. The findings may also contribute to our pathophysiological understanding of ulcerative colitis.

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