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Plasmacytoid dendritic cells do not migrate in intestinal or hepatic lymph.

Journal article
Authors Ulf Yrlid
Vuk Cerovic
Simon Milling
Christopher D Jenkins
Jiquan Zhang
Paul R Crocker
Linda S Klavinskis
G Gordon MacPherson
Published in Journal of immunology (Baltimore, Md. : 1950)
Volume 177
Issue 9
Pages 6115-21
ISSN 0022-1767
Publication year 2006
Published at Institute of Biomedicine, Department of Microbiology and Immunology
Pages 6115-21
Language en
Links www.ncbi.nlm.nih.gov/entrez/query.f...
Keywords Animals, Antigens, CD45, analysis, Cell Movement, Dendritic Cells, chemistry, drug effects, immunology, Histocompatibility Antigens Class II, analysis, Imidazoles, pharmacology, Intestines, immunology, Leukocytes, chemistry, immunology, Liver, immunology, Lymph, immunology, Lymphocyte Activation, Male, Oligodeoxyribonucleotides, pharmacology, Orthomyxoviridae, Rats, Rats, Inbred Strains, Receptors, Antigen, T-Cell, alpha-beta, analysis, Spleen, immunology, T-Lymphocytes, immunology, Thoracic Duct, immunology
Subject categories Microbiology in the medical area

Abstract

Plasmacytoid dendritic cells (pDCs) recognize pathogen-associated molecules, particularly viral, and represent an important mechanism in innate defense. They may however, also have roles in steady-state tolerogenic responses at mucosal sites. pDCs can be isolated from blood, mucosa, and lymph nodes (LNs). Although pDCs can express peripherally derived Ags in LNs and at mucosal sites, it is not clear whether pDCs actually migrate from the periphery in lymph or whether LN pDCs acquire Ags by other mechanisms. To determine whether pDCs migrate in lymph, intestine or liver-draining LNs were removed and thoracic duct leukocytes (TDLs) were collected. TDLs expressing MHC-II and CD45R, but not TCRalphabeta or CD45RA, were then analyzed. These enriched TDLs neither transcribe type I IFNs nor secrete inflammatory cytokines in response to viral stimuli in vitro or after a TLR7/8 stimulus in vivo. In addition, these TDLs do not express CD5, CD90, CD200, or Siglec-H, but do express Ig, and therefore represent B cells, despite their lack of CD45RA expression. Intestinal and hepatic lymph are hence devoid of bona fide pDCs under both steady-state conditions and after TLR7/8 stimulation. This shows that any role for pDCs in Ag-specific T cell activation or tolerance must differ from the roles of classical dendritic cells, because it cannot result from peripheral Ag capture, followed by migration of pDCs via lymph to the LN.

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