To the top

Page Manager: Webmaster
Last update: 9/11/2012 3:13 PM

Tell a friend about this page
Print version

The involvement of Aeromo… - University of Gothenburg, Sweden Till startsida
Sitemap
To content Read more about how we use cookies on gu.se

The involvement of Aeromonas salmonicida virulence factors in bacterial translocation across the rainbow trout, Oncorhynchus mykiss (Walbaum), intestine.

Journal article
Authors Fredrik Jutfelt
Henrik Sundh
J Glette
L Mellander
Björn Thrandur Björnsson
Kristina Sundell
Published in Journal of fish diseases
Volume 31
Issue 2
Pages 141-51
ISSN 0140-7775
Publication year 2008
Published at Department of Zoology
Pages 141-51
Language en
Links dx.doi.org/10.1111/j.1365-2761.2007...
Subject categories Animal physiology

Abstract

The pathogenic bacterium Aeromonas salmonicida is the causative agent of furunculosis, a lethal disease in salmonids. The mode of lateral transmission has not been conclusively defined, but A. salmonicida is able to translocate across the intestinal epithelium of salmonids, making the intestinal route a probable candidate. This study investigated some of the virulence mechanisms used by the bacteria to promote translocation. Intestinal segments were placed in modified Ussing chambers to investigate epithelial functions during exposure to bacterial factors. The factors were: extracellular products (ECP), lipopolysaccharide (LPS) or live or heat-inactivated A. salmonicida. Fluorescein isothiocynate (FITC)-labelling enabled detection of translocated bacteria by fluorometry. Live A. salmonicida translocated to a greater degree than heat-inactivated bacteria, suggesting that the bacteria utilize a heat sensitive surface-bound virulence factor which promotes translocation. The epithelium was negatively affected by ECP, manifested as decreased net ion transport, indicating a disturbance in ion channels or cell metabolism. LPS did not affect the epithelium in vitro when administered on the luminal side of the intestinal segment, but significantly increased epithelial translocation of fluorescent bacterial-sized microspheres when administered on the serosal side. This is suggested to be caused by increased transcellular transport, as the paracellular permeability was unaffected indicating maintained epithelial integrity.

Page Manager: Webmaster|Last update: 9/11/2012
Share:

The University of Gothenburg uses cookies to provide you with the best possible user experience. By continuing on this website, you approve of our use of cookies.  What are cookies?