To the top

Page Manager: Webmaster
Last update: 9/11/2012 3:13 PM

Tell a friend about this page
Print version

Changes in the ratio betw… - University of Gothenburg, Sweden Till startsida
To content Read more about how we use cookies on

Changes in the ratio between FPR and FPRL1 triggered superoxide production in human neutrophils-a tool in analysing receptor specific events

Journal article
Authors Huamei Fu
Jennie Karlsson
Lena Björkman
Anna-Lena Stenfeldt
Anna Karlsson
Johan Bylund
Claes Dahlgren
Published in Journal of Immunological Methods
Volume 331
Issue 1-2
Pages 50-8
ISSN 0022-1759
Publication year 2008
Published at Institute of Medicine, Department of Rheumatology and Inflammation Research
Institute of Biomedicine, Department of Microbiology and Immunology
Pages 50-8
Language en
Keywords Cyclooxygenase Inhibitors/pharmacology, HL-60 Cells, Humans, Neutrophils/drug effects/immunology/*metabolism, Oligopeptides/immunology/pharmacology, Piroxicam/pharmacology, Receptors, Formyl Peptide/agonists/immunology/*metabolism, Receptors, Lipoxin/agonists/immunology/*metabolism, Sodium Dodecyl Sulfate/pharmacology, Superoxides/*metabolism
Subject categories Medical and Health Sciences


Neutrophils express the G protein-coupled N-formyl peptide receptor (FPR) as well as its closely related homologue, formyl peptide like receptor 1 (FPRL1), and activation of these receptors induce a release of superoxide anions. The magnitude of the responses induced by the two peptide agonists fMLF and WKYMVM, specific for FPR and FPRL1, respectively, was found to be very variable in different neutrophil populations. The ratio between the FPR and FPRL1 triggered respiratory burst was, however, very constant and close to 1. The ratio was changed in neutrophils that were desensitized as well as when the signaling through either of the receptors was inhibited by receptor specific antagonists or by a PIP(2) binding peptide. The FPR/FPRL1 ratio was not changed in primed neutrophils or in differentiated HL-60 cells. We show that the change in the ratio, calculated from the amount of radical release in neutrophils triggered with FPR and FPRL1 specific agonists can be used as a valuable tool to find/identify receptor specific/selective changes mediated by peptides/proteins/drugs, as well as to identify cells from patients or groups of patients that diverge from normal cells in their FPR/FPRL1 triggered functions.

Page Manager: Webmaster|Last update: 9/11/2012

The University of Gothenburg uses cookies to provide you with the best possible user experience. By continuing on this website, you approve of our use of cookies.  What are cookies?