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The effect of external forces on discrete motion within holographic optical tweezers

Journal article
Authors Emma Eriksson
Stephen Keen
Jonathan Leach
Mattias Goksör
Miles Padgett
Published in Optics Express
Volume 15
Issue 26
Pages 18268-18274
ISSN 1094-4087
Publication year 2007
Published at Department of Physics (GU)
Pages 18268-18274
Language en
Subject categories Optics, Biophysics

Abstract

Holographic optical tweezers is a widely used technique to manipulate the individual positions of optically trapped micron-sized particles in a sample. The trap positions are changed by updating the holographic image displayed on a spatial light modulator. The updating process takes a finite time, resulting in a temporary decrease of the intensity, and thus the stiffness, of the optical trap. We have investigated this change in trap stiffness during the updating process by studying the motion of an optically trapped particle in a fluid flow. We found a highly nonlinear behavior of the change in trap stiffness vs. changes in step size. For step sizes up to approximately 300 nm the trap stiffness is decreasing. Above 300 nm the change in trap stiffness remains constant for all step sizes up to one particle radius. This information is crucial for optical force measurements using holographic optical tweezers.

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