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Contribution of AMPA and NMDA receptors to early and late phases of LTP in hippocampal slices.

Journal article
Authors Mikhail Dozmorov
Rui Li
Abdul-Karim Abbas
Fredrik Hellberg
Cecilia Farre
Fen-Sheng Huang
Barbro Jilderos
Holger Wigström
Published in Neuroscience research
Volume 55
Issue 2
Pages 182-8
ISSN 0168-0102
Publication year 2006
Published at Institute of Neuroscience and Physiology, Department of Physiology
Pages 182-8
Language en
Keywords Animals, Animals, Newborn, Excitatory Postsynaptic Potentials, drug effects, physiology, radiation effects, Hippocampus, drug effects, physiology, Long-Term Potentiation, drug effects, physiology, N-Methylaspartate, agonists, antagonists & inhibitors, pharmacology, Patch-Clamp Techniques, methods, Rats, Rats, Sprague-Dawley, Receptors, AMPA, physiology, Receptors, N-Methyl-D-Aspartate, physiology, Time Factors, alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid, agonists, antagonists & inhibitors, pharmacology
Subject categories Cell and molecular biology, Experimental brain research, Molecular neurobiology, Neurophysiology


Alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA) and N-methyl-D-aspartate (NMDA) receptor mediated responses were investigated in rat hippocampal slices under 4h of long-term potentiation (LTP) expression. A modified medium containing the NMDA receptor antagonist AP5 and low concentration of Mg(2+) was used to monitor isolated AMPA responses. NMDA components were determined from composite excitatory postsynaptic potentials (EPSPs) under brief (15-20 min) wash-out of AP5. LTP was induced in a medium with low concentration of AP5, resulting in an about two-fold larger increase of the AMPA component than of the NMDA component at both 1h and 4h after induction. Similar results were obtained if LTP was induced in "normal Mg(2+)" and the NMDA components were assessed at the end of experiment, from either composite or isolated NMDA EPSPs, with or without blockade of GABAergic inhibition. It is generally believed that LTP undergoes biochemical and/or structural conversions during the first few hours. Our study, however, shows constant expression of LTP, at least in terms of AMPA versus NMDA components, during this time. The data support the notion that LTP initiates as a predominant amplification of AMPA receptors and remains so for at least 4h.

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