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14-3-3 expression in denervated hippocampus after entorhinal cortex lesion assessed by culture-derived isotope tags in quantitative proteomics.

Journal article
Authors Carina Sihlbom
Ulrika Wilhelmsson
Lizhen Li
Carol L Nilsson
Milos Pekny
Published in Journal of proteome research
Volume 6
Issue 9
Pages 3491-500
ISSN 1535-3893
Publication year 2007
Published at Institute of Neuroscience and Physiology, Department of Clinical Neuroscience and Rehabilitation
Institute of Biomedicine, Department of Medical Biochemistry and Cell Biology
Pages 3491-500
Language en
Links dx.doi.org/10.1021/pr070108e
Keywords 14-3-3 Proteins, genetics, physiology, Animals, Astrocytes, metabolism, Cerebral Cortex, metabolism, Electrophoresis, Gel, Two-Dimensional, Entorhinal Cortex, pathology, Glial Fibrillary Acidic Protein, metabolism, Hippocampus, metabolism, Mass Spectrometry, methods, Mice, Mice, Inbred C57BL, Mice, Transgenic, Neurodegenerative Diseases, metabolism, Proteomics, methods, Vimentin, metabolism
Subject categories Neurobiology

Abstract

Activation of astrocytes accompanies many brain pathologies. Reactive astrocytes have a beneficial role in acute neurotrauma but later on might inhibit regeneration. 2D-gel electrophoresis and mass spectrometry were applied to study the proteome difference in denervated hippocampus in wildtype mice and mice lacking intermediate filament proteins glial fibrillary acidic protein (GFAP) and vimentin (GFAP-/-Vim-/-) that show attenuated reactive gliosis and enhanced posttraumatic regeneration. Proteomic data and immunohistochemical analyses showed upregulation of the adapter protein 14-3-3 four days postlesion and suggested that 14-3-3 upregulation after injury is triggered by reactive gliosis. Culture-derived isotope tags (CDIT) and mass spectrometry demonstrated that 14-3-3 epsilon was the major isoform upregulated in denervated hippocampus and that its upregulation was attenuated in GFAP-/-Vim-/- mice and thus most likely connected to reactive gliosis.

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